Solute Carrier Family 25 (Mitochondrial Carrier, Phosphate Carrier), Member 24 (SLC25A24) (AA 1-197) protein (His tag)

Details for Product No. ABIN3125044, Supplier: Log in to see
Protein Name
  • SLC25A24
  • 2610016M12Rik
  • apc1
  • scamc-1
  • scamc1-A
  • slc25a24
  • APC1
  • SCAMC-1
  • EFINAL
  • SCAMC1
  • calcium-binding mitochondrial carrier protein SCaMC-1-like
  • solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 24
  • solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 24
  • zgc:92470
  • LOC534742
  • Slc25a24
  • slc25a24-a
  • SLC25A24
  • zgc:92470
Protein Characteristics
AA 1-197
2
1
1
1
1
1
Origin
Mouse (Murine)
4
1
Source
Escherichia coli (E. coli)
2
2
1
Protein Type
Recombinant
Purification tag / Conjugate
This SLC25A24 protein is labelled with His tag.
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Sequence MLRWLRAFVL PTAACHDAEP PTRYETLFRA LDRNGDGVVD IGELQQGLQS LGIPLGQDAE EKIFTTGDVN KDGKLDFEEF MKYLKDHEKK MKLAFKSLDK NNDGKIEPSE IVQSLQMLGL HISEKQAELI LQSIDSDGTM TVDWNEWRDY FLFNPVTDIE EIIRFWKHST GIDIGDSLTI PDEFTEDEKK SGQWWRQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Characteristics
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Slc25a24 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
Purification Two step purification of proteins expressed in bacterial culture:
  1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
  2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Purity >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterility 0.22 μm filtered
Endotoxin Level Endotoxin has not been removed. Please contact us if you require endotoxin removal.
Grade Crystallography grade
Background Calcium-dependent mitochondrial solute carrier. Mediates the reversible, electroneutral exchange of Mg-ATP or Mg-ADP against phosphate ions, catalyzing the net uptake or efflux of adenine nucleotides across the mitochondrial inner membrane. Nucleotide transport is inactive when cytosolic calcium levels are low, and is activated by an increase in cytosolic calcium levels. May play a role in protecting cells against oxidative stress-induced cell death, probably by promoting the formation of calcium-phosphate precipitates in the mitochondrial matrix, and thereby buffering calcium levels in the mitochondrial matrix (By similarity). {ECO:0000250}.
Molecular Weight 23.7 kDa Including tag.
UniProt Q8BMD8
Application Notes In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Comment

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions For Research Use only
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -80 °C
Storage Comment Store at -80°C.
Expiry Date Unlimited (if stored properly)
Supplier Images
 image for Solute Carrier Family 25 (Mitochondrial Carrier, Phosphate Carrier), Member 24 (SLC25A24) (AA 1-197) protein (His tag) (ABIN3125044) „Crystallography Grade“ protein due to multi-step, protein-specific purification process
Did you look for something else?