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H1 Histone Family, Member N, Testis-Specific (H1FNT) (AA 1-398) protein (His tag)

Crystallography grade H1FNT Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, Crys, SDS, WB
Catalog No. ABIN3126131
  • Target See all H1 Histone Family, Member N, Testis-Specific (H1FNT) Proteins
    H1 Histone Family, Member N, Testis-Specific (H1FNT)
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-398
    Origin
    • 1
    • 1
    Mouse
    Source
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    His tag
    Application
    ELISA, Crystallization (Crys), SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    MAEAVQPSGE SQGAELTIQI QQPAERALRT PAKRGTQSVL RVSQLLLRAI AGHQHLTLDA LKKELGNAGY EVRREISSHH EGKSTRLEKG TLLRVSGSDA AGYFRVWKIS KPREKAGQSR LTLGSHSSGK TVLKSPRPLR PRSRRKAAKK AREVWRRKAR ALKARSRRVR TRSTSGARSR TRSRASSRAT SRATSRARSR ARSRAQSSAR SSARSSAKSS AKSSTRSSAK SWARSKARSR ARSRAKDLVR SKAREQAQAR EQARARAREQ AHARARTQDW VRAKAQEFVS AKEQQYVRAK EQERAKAREQ VRIGARDEAR IKAKDYNRVR PTKEDTSPRP AEEKSSNSKL REEKGQEPER PVKQTIQKPA LDNAPSIQGK ACTKSFTKSG QPGDTESP
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse H1fnt Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Grade
    Crystallography grade
    Top Product
    Discover our top product H1FNT Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    H1 Histone Family, Member N, Testis-Specific (H1FNT)
    Alternative Name
    H1fnt (H1FNT Products)
    Synonyms
    H1T2 Protein, 1700026P10Rik Protein, H1t2 Protein, Hanp1 Protein, mH1t2 Protein, H1 histone family member N, testis specific Protein, H1 histone family, member N, testis-specific Protein, H1FNT Protein, H1fnt Protein
    Background
    Essential for normal spermatogenesis and male fertility. Required for proper cell restructuring and DNA condensation during the elongation phase of spermiogenesis. Involved in the histone-protamine transition of sperm chromatin and the subsequent production of functional sperm. Binds both double-stranded and single-stranded DNA, ATP and protamine-1. {ECO:0000269|PubMed:15710904, ECO:0000269|PubMed:16055721}.
    Molecular Weight
    45.4 kDa Including tag.
    UniProt
    Q8CJI4
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