TAF6L Protein (AA 1-616) (Strep Tag)

Catalog No. ABIN3126678

Product Details

Target: TAF6L (TAF6-Like RNA Polymerase II, P300/CBP-Associated Factor (PCAF)-Associated Factor, 65kDa (TAF6L))

Protein Type: Recombinant

Protein Characteristics: AA 1-616

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This TAF6L protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MSEREERRFV EIPRESVRLM AESTGLELSD EVAALLAEDV CYRLREATQN SSQFMKHTKR RKLTVEDFNR ALRWSSVEAV CGYGSQEALP LRPAREGDLY FPEDREVSLV ELALATNIPK GCAETAVRVH VSYLDGKGNL APQGSVPSAV SSLTDDLLKY YQQVTRAVLG DDPQLMKVAL QDLQTNSKIA ALLPYFVYVV SGVKSVSHDL EQLHRLLQVA RSLIRNPHLC LGPYVRSLVG SVLYCVLEPL AASINPLNDH WTLRDGAALL LSHIFWTHGD LVSGLYQQIL LSLQKVLTDP VRPLCSHYGA VVGLHALGWK AVERVLYPHL PTYWTNLQAV LDDYSVSNAQ VKADGHKVYG AILVAVERLL KMKAQAAEPN RGGLGGRGYR PAEDLPWDSL LLQESPPGGS SETGFGSGLP PPSGVAGPED PSSLTLADIY RELYSFFGDS LATRFGTGQP APTAPRPPGD KKEPAAAPDS VRKMPQLTAS AVVSPQGDES PCGGTLATAT AASESRPLPR VHRARGAPRQ QGPGAGTRDV FQKSRFAPRG APHFRFIIAG RQAGRRCRGR LFQTAFPAPY GPSPASRYVQ KLPMIGRTGR PARRWALSDY SLYLPL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: TAF6L (TAF6-Like RNA Polymerase II, P300/CBP-Associated Factor (PCAF)-Associated Factor, 65kDa (TAF6L))

Alternative Name: Taf6l (TAF6L Products)

Synonyms: MGC80584 Protein, TAF6L Protein, 2810417N14Rik Protein, BB223262 Protein, C530024J06Rik Protein, Paf65a Protein, PAF65A Protein, TATA-box binding protein associated factor 6 like S homeolog Protein, TATA-box binding protein associated factor 6 like Protein, taf6l.S Protein, TAF6L Protein, Taf6l Protein

Background: TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L (TAF6L) (PCAF-associated factor 65-alpha) (PAF65-alpha),FUNCTION: Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex (By similarity). With TAF5L, acts as an epigenetic regulator essential for somatic reprogramming. Regulates target genes through H3K9ac deposition and MYC recruitment which trigger MYC regulatory network to orchestrate gene expression programs to control embryonic stem cell state (PubMed:31005419). Functions with MYC to activate target gene expression through RNA polymerase II pause release (PubMed:31005419). {ECO:0000250|UniProtKB:Q9Y6J9, ECO:0000269|PubMed:31005419}.

Molecular Weight: 67.2 kDa

UniProt: Q8R2K4