MTMR6 Protein (AA 1-617) (Strep Tag)

Catalog No. ABIN3127038

Product Details

Target: MTMR6 (Myotubularin Related Protein 6 (MTMR6))

Protein Type: Recombinant

Protein Characteristics: AA 1-617

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This MTMR6 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MEHIRTTKVE QVKLLDRFST NNKSLTGTLY LTATHLLFID AQQKETWILH HHIASVEKLA LTTSGCPLVI QCKNFRIVHF IVPRERDCHD IYNSLLQLSK QAKYEDLYAF SYNPKQNDTE RRNGWQLIDL AAEYERMGVP NANWQLSDAN REYKVCETYP RELYVPRTAS RPVIVGSSNF RSKGRLPVLS YCRQGTEAAI CRCSQPLSGF SARCLEDEHL LQAISKANPG NRYMYVVDTR PKLNAIANRA AGKGYENEDN YSNIRFQFVG IENIHVMRSS LQKLLEVNGS KGLSVNDFYS GLESSGWLRH IKAVLDAAIF LAKAIVVENA SVLVHCSDGW DRTSQVCSLG SLLLDSYYRT MKGFMVLIEK DWISFGHKFS ERCGHLDGDP REVSPVFTQF LECVWHLTQQ FPQAFEFNEA FLLQIHEHIH SCQFGNFLGN CQKEREELRL KEKTYSLWPF LLDDKKKYLN PLYSSKSQRL TVLEPNTASF NFKFWRNMYH QFDRTLHPRQ SVLSIIMNMN EQSKQLEEDI KDLEAKIKQC KNGILTKELL HAVHPESPAL KTSLCLKEPS LLPVKDTLRA IEGSSPADNR YCDYAEEFSK SEPTVVSLEY GVARMTC
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: MTMR6 (Myotubularin Related Protein 6 (MTMR6))

Alternative Name: Mtmr6 (MTMR6 Products)

Synonyms: 4022440C11Rik Protein, AI428804 Protein, AU041072 Protein, zgc:55289 Protein, wu:fi04a11 Protein, si:dkey-286f3.1 Protein, zgc:56405 Protein, myotubularin related protein 6 Protein, myotubularin related protein 8 Protein, MTMR6 Protein, Mtmr6 Protein, mtmr6 Protein, mtmr8 Protein

Background: Myotubularin-related protein 6 (Phosphatidylinositol-3,5-bisphosphate 3-phosphatase) (EC 3.1.3.95) (Phosphatidylinositol-3-phosphate phosphatase) (EC 3.1.3.64),FUNCTION: Phosphatase that acts on lipids with a phosphoinositol headgroup. Dephosphorylates phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 3,5-bisphosphate. Binds with high affinity to phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) but also to phosphatidylinositol 3-phosphate (PtdIns(3)P), phosphatidylinositol 4-phosphate (PtdIns(4)P), and phosphatidylinositol 5-phosphate (PtdIns(5)P), phosphatidic acid and phosphatidylserine (By similarity). Negatively regulates ER-Golgi protein transport (By similarity). Probably in association with MTMR9, plays a role in the late stages of macropinocytosis by dephosphorylating phosphatidylinositol 3-phosphate in membrane ruffles (PubMed:24591580). Acts as a negative regulator of KCNN4/KCa3.1 channel activity in CD4(+) T-cells possibly by decreasing intracellular levels of phosphatidylinositol 3-phosphate. Negatively regulates proliferation of reactivated CD4(+) T-cells. In complex with MTMR9, negatively regulates DNA damage-induced apoptosis. The formation of the MTMR6-MTMR9 complex stabilizes both MTMR6 and MTMR9 protein levels (By similarity). {ECO:0000250|UniProtKB:A0A0G2JXT6, ECO:0000250|UniProtKB:Q9Y217, ECO:0000269|PubMed:24591580}.

Molecular Weight: 70.9 kDa

UniProt: Q8VE11

Pathways: Inositol Metabolic Process