Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

TAF9 Protein (AA 1-264) (His tag)

Crystallography grade TAF9 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Catalog No. ABIN3127201
  • Target See all TAF9 Proteins
    TAF9 (RNA Polymerase II TBP-Associated Factor Subunit G (TAF9))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-264
    Origin
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    Mouse
    Source
    • 6
    • 2
    • 2
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This TAF9 protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
    Sequence
    MESGKMASPK SMPKDAQMMA QILKDMGITE YEPRVINQML EFAFRYVTTI LDDAKIYSSH AKKATVDADD VRLAIQCRAD QSFTSPPPRD FLLDIARQRN QTPLPLIKPY SGPRLPPDRY CLTAPNYRLK SLQKKAPAPA GRITVPRLSV GSVSSRPSTP TLGTPTPQTM SVSTKVGTPM SLTGQRFTVQ MPASQSPAVK ASIPATSTVQ NVLINPSLIG SKNILITTNM VSQNTAESAN ALKRKREDDD DDDDDDDDDD YDNM
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Taf9 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product TAF9 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    TAF9 (RNA Polymerase II TBP-Associated Factor Subunit G (TAF9))
    Alternative Name
    Taf9 (TAF9 Products)
    Synonyms
    AK6 Protein, CGI-137 Protein, CINAP Protein, CIP Protein, MGC:1603 Protein, MGC:3647 Protein, MGC:5067 Protein, TAF2G Protein, TAFII31 Protein, TAFII32 Protein, TAFIID32 Protein, hCINAP Protein, 2310012M09Rik Protein, 2810046E22Rik Protein, 4921516M08Rik Protein, AA673500 Protein, AU045423 Protein, Ak6 Protein, Taf2g Protein, im:7089279 Protein, wu:fb16h03 Protein, zgc:153410 Protein, TATA-box binding protein associated factor 9 Protein, adenylate kinase 6 L homeolog Protein, adenylate kinase isoenzyme 6-like Protein, SAGA complex/ transcription initiation factor Taf9 Protein, TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor Protein, TAF9 Protein, Taf9 Protein, ak6.L Protein, LOC100355082 Protein, taf9 Protein
    Background
    Essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. May have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription (By similarity). {ECO:0000250}.
    Molecular Weight
    29.9 kDa Including tag.
    UniProt
    Q8VI33
    Pathways
    Negative Regulation of intrinsic apoptotic Signaling
You are here:
Support