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Deiodinase, Iodothyronine, Type III (DIO3) (AA 1-304) protein (rho-1D4 tag)

Details for Product No. ABIN3127567, Supplier: Log in to see
Protein Name
  • 5DIII
  • DIOIII
  • txdi3
  • dioiii
  • XL-15
  • itdi3
  • D3
  • TXDI3
  • deiodinase, iodothyronine, type III
  • deiodinase, iodothyronine, type 3
  • deiodinase, iodothyronine type III
  • DIO3
  • dio3
  • Dio3
Protein Characteristics
AA 1-304
3
2
1
1
1
1
1
1
1
1
1
1
Origin
Mouse (Murine)
4
3
1
1
1
1
1
1
1
Source
Insect Cells
7
4
2
1
Protein Type
Recombinant
Purification tag / Conjugate
This DIO3 protein is labelled with rho-1D4 tag.
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
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Sequence MPRQAASRLV VGEGEGPPGA SGPAATMLRS LLLHSLRLCA QTASCLVLFP RFLGTAFMLW LLDFLCIRKH FLRRRHPDHP EPEVELNSEG EEMPPDDPPI CVSDDNRLCT LASLKAVWHG QKLDFFKQAH EGGPAPNSEV VRPDGFQSQR ILDYAQGTRP LVLNFGSCTU PPFMARMSAF QRLVTKYQRD VDFLIIYIEE AHPSDGWVTT DSPYVIPQHR SLEDRVSAAR VLQQGAPGCA LVLDTMANSS SSAYGAYFER LYVIQSGTIM YQGGRGPDGY QVSELRTWLE RYDEQLHGTR PHRF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Characteristics
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Dio3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
Purification Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
  1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
  2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
  3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Purity >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterility 0.22 μm filtered
Endotoxin Level Protein is endotoxin-free.
Grade Crystallography grade
Background Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development. {ECO:0000250|UniProtKB:P49897}.
Molecular Weight 35.3 kDa Including tag.
UniProt Q91ZI8
Research Area Endocrine system, Hormones, Cancer
Pathways Hormone Activity
Application Notes In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Comment

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions For Research Use only
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -80 °C
Storage Comment Store at -80°C.
Expiry Date Unlimited (if stored properly)
Supplier Images
 image for Deiodinase, Iodothyronine, Type III (DIO3) (AA 1-304) protein (rho-1D4 tag) (ABIN3127567) „Crystallography Grade“ protein due to multi-step, protein-specific purification process