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DNAJB11 Protein (AA 23-358) (His tag)

Crystallography grade DNAJB11 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Catalog No. ABIN3127952
  • Target See all DNAJB11 Proteins
    DNAJB11 (DnaJ (Hsp40) Homolog, Subfamily B, Member 11 (DNAJB11))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 23-358
    Origin
    • 3
    • 1
    • 1
    Mouse
    Source
    • 2
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This DNAJB11 protein is labelled with His tag.
    Application
    ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
    Sequence
    GRDFYKILGV PRSASIKDIK KAYRKLALQL HPDRNPDDPQ AQEKFQDLGA AYEVLSDSEK RKQYDTYGEE GLKDGHQSSH GDIFSHFFGD FGFMFGGTPR QQDRNIPRGS DIIVDLEVTL EEVYAGNFVE VVRNKPVARQ APGKRKCNCR QEMRTTQLGP GRFQMTQEVV CDECPNVKLV NEERTLEVEI EPGVRDGMEY PFIGEGEPHV DGEPGDLRFR IKVVKHRIFE RRGDDLYTNV TVSLVEALVG FEMDITHLDG HKVHISRDKI TRPGAKLWKK GEGLPNFDNN NIKGSLIITF DVDFPKEQLT EEAKEGIKQL LKQGPVQKVY NGLQGY
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Dnajb11 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Grade
    Crystallography grade
    Top Product
    Discover our top product DNAJB11 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    DNAJB11 (DnaJ (Hsp40) Homolog, Subfamily B, Member 11 (DNAJB11))
    Alternative Name
    Dnajb11 (DNAJB11 Products)
    Synonyms
    MGC75796 Protein, MGC81924 Protein, ABBP-2 Protein, ABBP2 Protein, DJ9 Protein, Dj-9 Protein, EDJ Protein, ERdj3 Protein, ERj3 Protein, ERj3p Protein, PRO1080 Protein, UNQ537 Protein, hDj-9 Protein, 1810031F23Rik Protein, AL024055 Protein, Dj9 Protein, LRRGT00084 Protein, cb954 Protein, DnaJ heat shock protein family (Hsp40) member B11 Protein, DnaJ heat shock protein family (Hsp40) member B11 S homeolog Protein, DnaJ (Hsp40) homolog, subfamily B, member 11 Protein, dnajb11 Protein, DNAJB11 Protein, dnajb11.S Protein, Dnajb11 Protein
    Background
    Serves as a co-chaperone for HSPA5. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity (By similarity). {ECO:0000250}.
    Molecular Weight
    39.1 kDa Including tag.
    UniProt
    Q99KV1
    Pathways
    ER-Nucleus Signaling
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