CPSF3L Protein (AA 1-600) (Strep Tag)

Catalog No. ABIN3128546

Product Details

Target: CPSF3L (Cleavage and Polyadenylation Specific Factor 3-Like (CPSF3L))

Protein Type: Recombinant

Protein Characteristics: AA 1-600

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This CPSF3L protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MPEIRVTPLG AGQDVGRSCI LVSISGKNVM LDCGMHMGYN DDRRFPDFSY ITQSGRLTDF LDCVIISHFH LDHCGALPYF SEMVGYDGPI YMTHPTQAIC PILLEDYRKI AVDKKGEANF FTSQMIKDCM KKVVAVHLHQ TVQVDDELEI KAYYAGHVLG AAMFQIKVGS ESVVYTGDYN MTPDRHLGAA WIDKCRPNLL ITESTYATTI RDSKRCRERD FLKKVHETVE RGGKVLIPVF ALGRAQELCI LLETFWERMN LKVPIYFSTG LTEKANHYYK LFITWTNQKI RKTFVQRNMF EFKHIKAFDR TFADNPGPMV VFATPGMLHA GQSLQIFRKW AGNEKNMVIM PGYCVQGTVG HKILSGQRKL EMEGRQMLEV KMQVEYMSFS AHADAKGIMQ LVGQAEPESV LLVHGEAKKM EFLRQKIEQE FRVSCYMPAN GETVTLPTSP SIPVGISLGL LKREMVQGLL PEAKKPRLLH GTLIMKDSNF RLVSSEQALK ELGLAEHQLR FTCRVHLQDT RKEQETALRV YSHLKSTLKD HCVQHLPDGS VTVESILIQA AAHSEDPGTK VLLVSWTYQD EELGSFLTTL LKNGLPQAPS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: CPSF3L (Cleavage and Polyadenylation Specific Factor 3-Like (CPSF3L))

Alternative Name: Ints11 (CPSF3L Products)

Synonyms: DDBDRAFT_0204486 Protein, DDBDRAFT_0234100 Protein, DDB_0204486 Protein, DDB_0234100 Protein, int11 Protein, CPSF73L Protein, INT11 Protein, INTS11 Protein, RC-68 Protein, RC68 Protein, 2410006F12Rik Protein, AU044035 Protein, RGD1306841 Protein, zgc:110671 Protein, CPSF3L Protein, DKFZp469C1432 Protein, cleavage and polyadenylation specific factor 3-like Protein, integrator complex subunit 11 Protein, RNA-metabolising metallo-beta-lactamase domain-containing protein Protein, microRNA 6727 Protein, CPSF3L Protein, INTS11 Protein, ints11 Protein, Ints11 Protein, MIR6727 Protein

Background: Integrator complex subunit 11 (Int11) (EC 3.1.27.-) (Cleavage and polyadenylation-specific factor 3-like protein) (CPSF3-like protein),FUNCTION: Catalytic component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates the snRNAs 3' cleavage. Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the INT complex. {ECO:0000250|UniProtKB:Q5TA45}.

Molecular Weight: 67.8 kDa

UniProt: Q9CWS4