GARS Protein (AA 1-729) (His tag)

Catalog No. ABIN3128710

Product Details

Target: GARS (Glycyl-tRNA Synthetase (GARS))

Protein Type: Recombinant

Protein Characteristics: AA 1-729

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This GARS protein is labelled with His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequence: MPCLLPSLLR ATRAALPLLS PPRVVAASAS QRLLSAPAQP AASRSSMDSA EELLAPLRLA VRQQGDFVRK LKEDKAPQVD VDRAVAELKA RKRVLEAKEL ALQPKDDIVD RAKMEDTLKR RFFYDQAFAI YGGVSGLYDF GPVGCALKNN IIQAWRQHFI QEEQILEIDC TMLTPEPVLK TSGHVDKFAD FMVKDVKNGE CFRADHLLKA HLQKLMSDKK CSAEKKSEME SVLAQLDNYG QQELADLFVN YNVKSPTTGN DLSPPVPFNL MFQTFIGPGG NMPGYLRPET AQGIFLNFKR LLEFNQGKLP FAAAQIGNSF RNEISPRSGL IRVREFTMAE IEHFVDPTEK DHPKFQSVAD LCLYLYSAKA QVTGQSARKM RLGDAVEQGV INNSVLGYFI GRIYLYLTKV GISPDKLRFR QHMENEMAHY ACDCWDAESK TSYGWIEIVG CADRSCYDLS CHARATKVPL VAEKPLKEPK TVNVVQFEPN KGAVGKAYKK DAKLVLEYLS ACDECYISEM ELLLSEKGEF TIETEGKTFQ LTKDMVSVKR FQKTLHVEEV VPSVIEPSFG LGRIMYTILE HTFHVREGDE QRTFFSFPAV VAPFKCSVLP LSQNQEFMPF VKELSEALTR NGVSHKVDDS SGSIGRRYAR TDEIGVAFGI TIDFDTVNKT PHTATLRDRD SMRQIRAEVS ELPNVVRDLA NGNITWADVE ARYPLFEGQE TGKKETVEE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Gars Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: GARS (Glycyl-tRNA Synthetase (GARS))

Alternative Name: Gars (GARS Products)

Synonyms: CMT2D Protein, DSMAV Protein, GlyRS Protein, HMN5 Protein, SMAD1 Protein, Aat-gly Protein, CG6778 Protein, Dmel\\CG6778 Protein, GRS Protein, gars Protein, team Protein, RGD1559871 Protein, GENA202 Protein, Gena201 Protein, Nmf249 Protein, Sgrp23 Protein, GB13467 Protein, MGC79495 Protein, fb02f03 Protein, si:dkey-276i5.1 Protein, wu:fb02f03 Protein, NI36_08435 Protein, glycyl-tRNA synthetase Protein, Glycyl-tRNA synthetase Protein, glycine--tRNA ligase Protein, glycyl-tRNA synthetase L homeolog Protein, Glycine--tRNA ligase Protein, GARS Protein, GlyRS Protein, Gars Protein, LOC408392 Protein, gars.L Protein, gars Protein, LOC692991 Protein, glyQ Protein, TTHA0543 Protein, NI36_RS08405 Protein

Background: Catalyzes the attachment of glycine to tRNA(Gly). Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs (By similarity). {ECO:0000250|UniProtKB:P41250}.

Molecular Weight: 82.8 kDa Including tag.

UniProt: Q9CZD3

Pathways: Ribonucleoside Biosynthetic Process