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SFRS9 Protein (AA 1-222) (His tag)

Crystallography grade SFRS9 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Catalog No. ABIN3128777
  • Target See all SFRS9 Proteins
    SFRS9 (serine/arginine-Rich Splicing Factor 9 (SFRS9))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-222
    Origin
    Mouse
    Source
    • 2
    • 2
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This SFRS9 protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
    Sequence
    MSSGWADERG GEGDGRIYVG NLPSDVREKD LEDLFYKYGR IREIELKNRH GLVPFAFVRF EDPRDAEDAI YGRNGYDYGQ CRLRVEFPRT YGGRGGWPRG ARNGPPTRRS DFRVLVSGLP PSGSWQDLKD HMREAGDVCY ADVQKDGMGM VEYLRKEDME YALRKLDDTK FRSHEGETSY IRVYPERSTS YGYSRSRSGS RGRDSPYQSR GSPHYFSPFR PY
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Srsf9 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Grade
    Crystallography grade
    Top Product
    Discover our top product SFRS9 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    SFRS9 (serine/arginine-Rich Splicing Factor 9 (SFRS9))
    Alternative Name
    Srsf9 (SFRS9 Products)
    Synonyms
    SFRS9 Protein, SRp30c Protein, 25kDa Protein, 2610029M16Rik Protein, Sfrs9 Protein, sfrs9 Protein, srp30c Protein, zgc:77449 Protein, serine and arginine rich splicing factor 9 Protein, serine/arginine-rich splicing factor 9 Protein, serine/arginine-rich splicing factor 9 L homeolog Protein, SRSF9 Protein, Srsf9 Protein, srsf9.L Protein, srsf9 Protein
    Background
    Plays a role in constitutive splicing and can modulate the selection of alternative splice sites. Represses the splicing of MAPT/Tau exon 10 (By similarity). {ECO:0000250}.
    Molecular Weight
    26.6 kDa Including tag.
    UniProt
    Q9D0B0
    Pathways
    Ribonucleoprotein Complex Subunit Organization
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