DIMT1 Protein (AA 1-313) (Strep Tag)

Catalog No. ABIN3128784

Product Details

Target: DIMT1 (DIM1 Dimethyladenosine Transferase 1 Homolog (DIMT1))

Protein Type: Recombinant

Protein Characteristics: AA 1-313

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This DIMT1 protein is labelled with Strep Tag.

Application: SDS-PAGE (SDS), ELISA, Western Blotting (WB)

Sequence: MPKAKSAASS RRRDRQEQRR ELKRAGGLMF NTGIGQHILK NPLIVNSIID KAALRPTDVV LEVGPGTGNM TVKLLEKAKK VVACELDPRL VAELHKRVQG TPLASKLQVL VGDVLKSDLP FFDACVANLP YQISSPFVFK LLLHRPFFRC AILMFQREFA LRLVAKPGDK LYCRLSINTQ LLARVDHLMK VGKNNFRPPP KVESSVVRIE PKNPPPPINF QEWDGLVRIT FVRKNKTLSA AFKSSAVQQL LEKNYRIHCS VQNTVIPEDF SIADKIQQIL TSTGFSDKRA RSMDIDDFIR LLHGFNAEGI HFS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: DIMT1 (DIM1 Dimethyladenosine Transferase 1 Homolog (DIMT1))

Alternative Name: Dimt1 (DIMT1 Products)

Synonyms: wu:fc96g04 Protein, zgc:101122 Protein, DIMT1 Protein, HSA9761 Protein, DIM1 Protein, DIMT1L Protein, HUSSY5 Protein, Dimt1l Protein, RGD1311752 Protein, 1500031M22Rik Protein, AV111347 Protein, DIM1 dimethyladenosine transferase 1 homolog Protein, DIM1 dimethyladenosine transferase 1-like (S. cerevisiae) Protein, dimethyladenosine transferase Protein, 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase Protein, DIMT1 Protein, dimt1l Protein, Mrub_1171 Protein, MMAH_RS05170 Protein, Arnit_0151 Protein, Ndas_5229 Protein, Mesil_2614 Protein, Arch_1591 Protein, Spirs_1508 Protein, Deba_0940 Protein, Olsu_1437 Protein, Toce_0031 Protein, Acear_0057 Protein, MPET_RS09405 Protein, VDIS_RS12100 Protein, Saut_2148 Protein, Fbal_3362 Protein, Ilyop_1321 Protein, MFER_RS05130 Protein, Calni_0771 Protein, Ocepr_1809 Protein, Dimt1 Protein

Background: Probable dimethyladenosine transferase (EC 2.1.1.183) (DIM1 dimethyladenosine transferase 1 homolog) (DIM1 dimethyladenosine transferase 1-like) (Probable 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase) (Probable 18S rRNA dimethylase) (Probable S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase),FUNCTION: Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle. Involved in the pre-rRNA processing steps leading to small-subunit rRNA production independently of its RNA-modifying catalytic activity. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. {ECO:0000250|UniProtKB:Q9UNQ2}.

Molecular Weight: 35.3 kDa

UniProt: Q9D0D4