U2AF1 Protein (AA 2-239) (His tag)

Catalog No. ABIN3129296

Product Details

Target: U2AF1 (U2 Small Nuclear RNA Auxiliary Factor 1 (U2AF1))

Protein Type: Recombinant

Protein Characteristics: AA 2-239

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This U2AF1 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequence: AEYLASIFGT EKDKVNCSFY FKIGACRHGD RCSRLHNKPT FSQTIALLNI YRNPQNSSQS ADGLRCAVSD VEMQEHYDEF FEEVFTEMEE KYGEVEEMNV CDNLGDHLVG NVYVKFRREE DAEKAVIDLN NRWFNGQPIH AELSPVTDFR EACCRQYEMG ECTRGGFCNF MHLKPISREL RRELYGRRRK KHRSRSRSRE RRSRSRDRGR GGGGGGGGGG GRERDRRRSR DRERSGRF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse U2af1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: U2AF1 (U2 Small Nuclear RNA Auxiliary Factor 1 (U2AF1))

Alternative Name: U2af1 (U2AF1 Products)

Synonyms: DDBDRAFT_0218665 Protein, DDBDRAFT_0233354 Protein, DDB_0218665 Protein, DDB_0233354 Protein, 2010107D16Rik Protein, 35kDa Protein, FP793 Protein, RN Protein, RNU2AF1 Protein, U2AF35 Protein, U2AFBP Protein, CHUNP6860 Protein, zgc:111896 Protein, U2 small nuclear RNA auxiliary factor 1 Protein, CCHC-type zinc finger-containing protein Protein, splicing factor U2AF 35 kDa subunit Protein, U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1 Protein, U2 small nuclear RNA auxiliary factor 1 S homeolog Protein, U2AF1 Protein, u2af1 Protein, LOC737227 Protein, U2af1 Protein, u2af1.S Protein

Background: Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron (By similarity). {ECO:0000250}.

Molecular Weight: 28.6 kDa Including tag.

UniProt: Q9D883