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PDILT Protein (AA 21-588) (His tag)

Crystallography grade PDILT Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Catalog No. ABIN3129486
  • Target See all PDILT Proteins
    PDILT (Protein Disulfide Isomerase-Like, Testis Expressed (PDILT))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 21-588
    Origin
    Mouse
    Source
    • 2
    • 2
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This PDILT protein is labelled with His tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Sequence
    SPEIDTGINI SQPLHILEDH NLMVLTPAGL TQTLNETRFL MVIFHNPSLK QSRKLAKELG KAAEIFGKGK NGLGFGKVDI TKETELQQEF DITHAPELKL FFEGNRLKPI SCKDVVESTA LVVWLRRQIS KKALLFNNSD EVADFVKSRP LVIVGFFQDL EEEVAELFYD TIKDFPELTF GAIQIKNSFG RFHVILDSVL VFKKGKIVKR QELINDSTNK DHLNQVIKQQ LTGFVIELNP ENKDLIYELN ILNHMLLFIS KSSEPYSTIS RHYRQIAKEF QNKILFVLVN ADEPKNKRIF EYFQISRVNV PSVQILNLSS DGRYKMPTDD INFESLKKFC NSFLSKTAKK HKASEEIPKY WDQGPVKKLV GKNFNVVVLD KEKDVFVMFY APWSEKCRVL LPLLEELGIK YQNHSTVIIA KIDITANDIQ LANPEQYPFF RLFPTDSQEA VMYKGEHTMK GFCDFLESHV KVRIEEEDEL LYIEQNEEEE VLAEPEIQLI EKLPENPLLK IEDTSKQDRP VKESPVLDSI RKPEEPERRK ETAEREAAAA QPKEQPKPER KLEVKEEL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Pdilt Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Grade
    Crystallography grade
    Top Product
    Discover our top product PDILT Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    PDILT (Protein Disulfide Isomerase-Like, Testis Expressed (PDILT))
    Alternative Name
    Pdilt (PDILT Products)
    Synonyms
    1700007B13Rik Protein, RGD1307822 Protein, PDIA7 Protein, protein disulfide isomerase-like, testis expressed Protein, protein disulfide isomerase like, testis expressed Protein, Pdilt Protein, PDILT Protein
    Background
    Probable redox-inactive chaperone involved in spermatogenesis. {ECO:0000250}.
    Molecular Weight
    66.6 kDa Including tag.
    UniProt
    Q9DAN1
    Pathways
    Cell RedoxHomeostasis
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