GLCE Protein (AA 30-618) (His tag)

Catalog No. ABIN3129756

Product Details

Target: GLCE (Glucuronic Acid Epimerase (GLCE))

Protein Type: Recombinant

Protein Characteristics: AA 30-618

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This GLCE protein is labelled with His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequence: KCSSDKAIQF PRHLSSGFRV DGLEKRSAAS ESNHYANHIA KQQSEEAFPQ EQQKAPPVVG GFNSNGGSKV LGLKYEEIDC LINDEHTIKG RREGNEVFLP FTWVEKYFDV YGKVVQYDGY DRFEFSHSYS KVYAQRSPYH PDGVFMSFEG YNVEVRDRVK CISGVEGVPL STQWGPQGYF YPIQIAQYGL SHYSKNLTEK PPHIEVYETA EDRDRNIRPN EWTVPKGCFM ASVADKSRST NVKQFIAPET SEGVSLQLGN TKDFIISFDL KLLTNGSVSV VLETTEKNQL FTVHYVSNTQ LIAFRDRDIY YGIGPRTSWS TVTRDLVTDL RKGVGLSNTK AVKPTKIMPK KVVRLIAKGK GFLDNITIST TAHMAAFFAA SDWLVRNQDE KGGWPIMVTR KLGEGFKSLE PGWYSAMAQG QAISTLVRAY LLTKDYVFLS SALRATAPYK FPSEQHGVKA VFMNKHDWYE EYPTTPSSFV LNGFMYSLIG LYDLKETAGE TLGKEARSLY ERGMESLKAM LPLYDTGSGT IYDLRHFMLG IAPNLARWDY HTTHINQLQL LSTIDESPIF KEFVKRWKSY LKGSRAKHN
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Glce Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: GLCE (Glucuronic Acid Epimerase (GLCE))

Alternative Name: Glce (GLCE Products)

Synonyms: HSEPI Protein, RGD1565253 Protein, MGC79110 Protein, 1110017N23Rik Protein, AI747411 Protein, C130034A12Rik Protein, Hsepi Protein, glce Protein, glcel Protein, sb:eu394 Protein, zgc:136701 Protein, glucuronic acid epimerase Protein, glucuronic acid epimerase L homeolog Protein, glucuronyl C5-epimerase Protein, glucuronic acid epimerase b Protein, glucuronic acid epimerase a Protein, GLCE Protein, Glce Protein, glce.L Protein, glce Protein, glceb Protein, glcea Protein

Background: Converts D-glucuronic acid residues adjacent to N-sulfate sugar residues to L-iduronic acid residues, both in maturing heparan sulfate (HS) and heparin chains. This is important for further modifications that determine the specificity of interactions between these glycosaminoglycans and proteins. {ECO:0000269|PubMed:11274177, ECO:0000269|PubMed:11279150, ECO:0000269|PubMed:16532012, ECO:0000269|PubMed:20208005}.

Molecular Weight: 67.7 kDa Including tag.

UniProt: Q9EPS3

Pathways: Glycosaminoglycan Metabolic Process