DNAJC7 Protein (AA 2-494) (His tag)

Catalog No. ABIN3130530

Product Details

Target: DNAJC7 (DnaJ (Hsp40) Homolog, Subfamily C, Member 7 (DNAJC7))

Protein Type: Recombinant

Protein Characteristics: AA 2-494

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This DNAJC7 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: AATAECDVVM AATEPELLED EDAKREAESF KEQGNAYYAK KDYNEAYNYY TKAIDMCPNN ASYYGNRAAT LMMLGRFREA LGDAQQSVRL DDSFVRGHLR EGKCHLSLGN AMAACRSFQR ALELDHKNAQ AQQEFKNANA VMEYEKIAEV DFEKRDFRKV VFCMDRALEF APACHRFKIL KAECLAMLGR YPEAQFVASD ILRMDSTNAD ALYVRGLCLY YEDCIEKAVQ FFVQALRMAP DHEKACVACR NAKALKAKKE DGNKAFKEGN YKLAYELYTE ALGIDPNNIK TNAKLYCNRG TVNSKLRQLE DAIEDCTNAV KLDDTYIKAY LRRAQCYMDT EQFEEAVRDY EKVYQTEKTK EHKQLLKNAQ LELKKSKRKD YYKILGVDKN ASEDEIKKAY RKRALMHHPD RHSGASAEVQ KEEEKKFKEV GEAFTILSDP KKKTRYDSGQ DLDEEGMNMG DFDANNIFKA FFGGPGGFSF EASGPGNFYF QFG
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Dnajc7 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: DNAJC7 (DnaJ (Hsp40) Homolog, Subfamily C, Member 7 (DNAJC7))

Alternative Name: Dnajc7 (DNAJC7 Products)

Synonyms: CCRP Protein, dnajc7 Protein, MGC89403 Protein, BEST:LD24941 Protein, CG4599 Protein, Dmel\\CG4599 Protein, EU3220 Protein, Tpr-2 Protein, Trp2 Protein, dTPR2 Protein, dTpr2 Protein, dtpr2 Protein, dtrpr2 Protein, tpr2 Protein, DJ11 Protein, DJC7 Protein, TPR2 Protein, TTC2 Protein, 2010003F24Rik Protein, 2010004G07Rik Protein, Ttc2 Protein, mDj11 Protein, mTpr2 Protein, wu:fj58b08 Protein, zgc:85806 Protein, DnaJ heat shock protein family (Hsp40) member C7 Protein, Tetratricopeptide repeat protein 2 Protein, DnaJ (Hsp40) homolog, subfamily C, member 7 Protein, Dnajc7 Protein, DNAJC7 Protein, dnajc7 Protein, Tpr2 Protein

Background: Acts as co-chaperone regulating the molecular chaperones HSP70 and HSP90 in folding of steroid receptors, such as the glucocorticoid receptor and the progesterone receptor. Proposed to act as a recycling chaperone by facilitating the return of chaperone substrates to early stages of chaperoning if further folding is required. In vitro, induces ATP-independent dissociation of HSP90 but not of HSP70 from the chaperone-substrate complexes (By similarity). Recruits NR1I3 to the cytoplasm. {ECO:0000250}.

Molecular Weight: 57.3 kDa Including tag.

UniProt: Q9QYI3