NXT1 Protein (AA 2-140) (His tag)

Catalog No. ABIN3130634

Product Details

Target: NXT1 (NTF2-Related Export Protein 1 (NXT1))

Protein Type: Recombinant

Protein Characteristics: AA 2-140

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This NXT1 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequence: ASVDFKTYVD QACRAAEEFV NVYYTTMDKR RRLLSRLYMG TATLVWNGNA VSGQESLSEF FEMLPSSEFQ ISVVDCQPVH DDATPSQTTV LVVICGTVKF EGNKQRDFNQ NFILTAQASP SNTVWKIASD CFRFQDWAS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Nxt1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: NXT1 (NTF2-Related Export Protein 1 (NXT1))

Alternative Name: Nxt1 (NXT1 Products)

Synonyms: CG12752 Protein, DmNXT1 Protein, Dmel\\CG12752 Protein, Nxt Protein, dNxt Protein, dmNXT Protein, dmNXT1 Protein, dmP15 Protein, nxt1 Protein, p15 Protein, GB14514 Protein, MTR2 Protein, P15 Protein, 1110001N02Rik Protein, NTF2-related export protein 1 Protein, NTF2-related export protein Protein, nuclear transport factor 2 like export factor 1 Protein, nuclear transport factor 2-like export factor 1 Protein, Nxt1 Protein, LOC413378 Protein, Tsp_05214 Protein, NXT1 Protein

Background: Stimulator of protein export for NES-containing proteins. Also plays a role in the nuclear export of U1 snRNA, tRNA, and mRNA. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 (By similarity). {ECO:0000250}.

Molecular Weight: 16.7 kDa Including tag.

UniProt: Q9QZV9