L3MBTL1 Protein (AA 1-826) (Strep Tag)

Catalog No. ABIN3130678

Product Details

Target: L3MBTL1 (L(3)mbt-Like 1 (L3MBTL1))

Protein Type: Recombinant

Protein Characteristics: AA 1-826

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This L3MBTL1 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MEGHTDMEIL RTVKGSSTGE VNVHLVARDS AGPHPQLPTT AFIIPTNAAT LGLPSTALDV PYPREPVHVG ALERVAGSEP VTATILPQLS TGTGTNSTVR LLDWTGVSAP LPGSGMRFRI NEYAPLNMIG VERPRSPEQR HEGGMARRDA GIQHPDVHQD RQDITSLEPP VDASSCKCQA CGPQQSSGLD VGSSGDRCSQ PFQKRSVIVE NSGCTIASEL LKPMKKRKHK EYQSPSEESE PEAVKQGEGK DAEREPTPST PENEEWSRSQ LVSSEKKDGW SWESYLEEQK AVTAPVSLFQ DSQAVTHNKN GFKLGMKLEG IDPQHPSMYF ILTVAEVCGY RLRLHFDGYS ECHDFWVNAN SPDIHPAGWF EKTGHKLQLP KGYKEEEFSW SQYLRSTKAQ AAPKHLFVSQ SHSTPPVGFQ VGMKLEAVDR MNPSLVCVAS VTDVVDSRFL VHFDDWGDTY DYWCDPSSPY IHPVGWCQKQ GKPLTPPQDY PDPDSFCWEK YLEETGTSAV PNWAFKVRPP HSFLVNMKLE AVDRRNPALI RVASVEDVED HRIKLHFDGW SHNYDFWIDA DHPDIHPAGW CSKTGHPLEP PLRPRESSSV SPGGCPPLSH RSPPHTKTSK YNFHHRKCPT PGCDGSGHVT GKFTAHHCLS GCPLAEKNQS RLKAELSDSE TAARKKNPSN LSPRKKPRHQ GRIGRPPKYR KIPEEDLQAL PPSVVHQSLF MSTLPTHADR PLSVCWEQHC KLLPGVAGIS ASTVSKWTIE EVFGFVQTLT GSEDQARLFK DEMIDGEAFL LLTQADIVKI MSVKLGPALK IYNAILMFKN TDDAFK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: L3MBTL1 (L(3)mbt-Like 1 (L3MBTL1))

Alternative Name: L3mbtl1 (L3MBTL1 Products)

Synonyms: C630004G01 Protein, L3mbtl Protein, mKIAA0681 Protein, H-L(3)MBT Protein, L3MBTL Protein, ZC2HC3 Protein, dJ138B7.3 Protein, RGD1307316 Protein, l(3)mbt-like (Drosophila) Protein, L3MBTL1, histone methyl-lysine binding protein Protein, l(3)mbt-like 1 (Drosophila) Protein, L3mbtl1 Protein, L3MBTL1 Protein

Background: Lethal(3)malignant brain tumor-like protein 1 (H-l(3)mbt) (H-l(3)mbt protein) (L(3)mbt-like) (L(3)mbt protein homolog),FUNCTION: Polycomb group (PcG) protein that specifically recognizes and binds mono- and dimethyllysine residues on target proteins, therey acting as a 'reader' of a network of post-translational modifications. PcG proteins maintain the transcriptionally repressive state of genes: acts as a chromatin compaction factor by recognizing and binding mono- and dimethylated histone H1b/H1-4 at 'Lys-26' (H1bK26me1 and H1bK26me2) and histone H4 at 'Lys-20' (H4K20me1 and H4K20me2), leading to condense chromatin and repress transcription. Recognizes and binds p53/TP53 monomethylated at 'Lys-382', leading to repress p53/TP53-target genes. Also recognizes and binds RB1/RB monomethylated at 'Lys-860'. Participates in the ETV6-mediated repression. Probably plays a role in cell proliferation. Overexpression induces multinucleated cells, suggesting that it is required to accomplish normal mitosis (By similarity). {ECO:0000250}.

Molecular Weight: 91.8 kDa

UniProt: A2A5N8

Pathways: Chromatin Binding