SAFB Protein (AA 2-937) (His tag)

Catalog No. ABIN3131089

Product Details

Target: SAFB (Scaffold Attachment Factor B (SAFB))

Protein Type: Recombinant

Protein Characteristics: AA 2-937

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This SAFB protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: AETLSGLGDA SAAGAAAVSS AASETGTRRL SDLRVIDLRA ELKKRNLDSS GNKSVLMERL KKAIEDEGGN PDEIEVTSEC NKKMPKRPSK GRKPEDEGVE DNGLEENSGD GQEDVETSLE NLQDMDMMDI SVLDEADIDN GSVADCVEEE EEATLPEGLA DSTELVEGDL KGLPEQLQEH AIDDKDTVNN VDTSSSDFTM LQEMEEASLE PENEKILDIL GETCKSEPVK EEGSELEQPF AQATSSVGPD RKLAEEEDLF ESCGHPEEEE EEEEEDQEEE QEEEGDLALA SSSKSESPST RCQWSEADAP LAVVKREPAD APGGGTGMDR EPVGLEEPVE QSSTAAQLPE ATSQELVRAP TAALSPEPQD SKEDVKKFAF DACNDVPAPP KESSASEGAD QKMSSVEEDS DTKRLSREEK GRSSCGRNFW VSGLSSTTRA TDLKNLFSRY GKVVGAKVVT NARSPGARCY GFVTMSTAEE ATKCISHLHK TELHGKMISV EKAKSEPTGK RVPDRRDGDS KKEKASTSDR SANLKREEKG ERKDDAKKTD DGSTEKSKDA DDQKPGPSER SRTTKSGSRG TERTVVMDKS KGVPVISVKT SGSKERASKS QDRKSASREK RSVVSFDKVK ESRKSRDSES RRERERSERE QRLQAQWERE ERERLEIARE RLAFHRHRLE RERMERERLE RERMHVEQER RREQERIHRE REELRRQQEL RYEQERRPAV RRPYEVDGRR DDAYWPEAKR AALDDRYHSD FSRQDRFHDF DHRDRGRYPN HSVDRREGSR SMMGDREGQH YPERHGGPER HGRDSRDGWG YGSNKRLSEG RGLPPPPRRD WGEHGRRLED DRAWQGTADG GMMERDHKRW QGGERSMSGH SGPGHMMNRG GMSGRGSFAP GGASRGHVIP RGGMQAGFGG QSRGSRPSDA RFTRRY
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Safb Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SAFB (Scaffold Attachment Factor B (SAFB))

Alternative Name: Safb (SAFB Products)

Synonyms: HAP Protein, HET Protein, SAF-B1 Protein, SAFB1 Protein, 3110021E02Rik Protein, 5330423C17Rik Protein, AU018122 Protein, D18386 Protein, E130307D12 Protein, zgc:55387 Protein, wu:fb40a03 Protein, wu:fd42g06 Protein, SAFB2 Protein, SAFB Protein, scaffold attachment factor B Protein, scaffold attachment factor B S homeolog Protein, SAFB-like transcription modulator Protein, Scaffold attachment factor B Protein, SAFB Protein, Safb Protein, safb Protein, safb.S Protein, LOC5570070 Protein, CpipJ_CPIJ017179 Protein, Bm1_49330 Protein, LOAG_00080 Protein

Background: Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing. Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. Can inhibit cell proliferation. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter. {ECO:0000269|PubMed:19403048}.

Molecular Weight: 105.9 kDa Including tag.

UniProt: D3YXK2

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway