NLRP12 Protein (AA 1-1054) (Strep Tag)

Catalog No. ABIN3131152

Product Details

Target: NLRP12 (NLR Family, Pyrin Domain Containing 12 (NLRP12))

Protein Type: Recombinant

Protein Characteristics: AA 1-1054

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This NLRP12 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MLPSTARDGL YRLSTYLEEL EAGELKKFKL FLGIAEDLSQ DKIPWGRMEK AGPLEMAQLM VAHMGTREAW LLALSTFQRI HRKDLWERGQ GEDLVRVTPN NGLCLFESQS ACPLDVSPNA PRKDLQTTYK DYVRRKFQLM EDRNARLGEC VNLSNRYTRL LLVKEHSNPI WTQQKFVDVE WERSRTRRHQ TSPIQMETLF EPDEERPEPP HTVVLQGAAG MGKSMLAHKV MLDWADGRLF QGRFDYVFYI SCRELNRSHT QCSVQDLISS CWPERGISLE DLMQAPDRLL FIIDGFDKLH PSFHDAQGPW CLCWEEKQPT EVLLGSLIRR LLLPQVSLLI TTRPCALEKL HGLLEHPRHV EILGFSEEAR KEYFYRYFHN TGQASRVLSF LMDYEPLFTM CFVPMVSWVV CTCLKQQLES GELLRQTPRT TTAVYMFYLL SLMQPKPGTP TFKVPANQRG LVSLAAEGLW NQKILFDEQD LGKHGLDGAD VSTFLNVNIF QKGIKCEKFY SFIHLSFQEF FAAMYCALNG REAVRRALAE YGFSERNFLA LTVHFLFGLL NEEMRCYLER NLGWSISPQV KEEVLAWIQN KAGSEGSTLQ HGSLELLSCL YEVQEEDFIQ QALSHFQVVV VRSISTKMEH MVCSFCARYC RSTEVLHLHG SAYSTGMEDD PPEPSGVQTQ STYLQERNML PDVYSAYLSA AVCTNSNLIE LALYRNALGS QGVRLLCQGL RHASCKLQNL RLKRCQISGS ACQDLAAAVI ANRNLIRLDL SDNSIGVPGL ELLCEGLQHP RCRLQMIQLR KCLLEAAAGR SLASVLSNNS YLVELDLTGN PLEDSGLKLL CQGLRHPVCR LRTLWLKICH LGQASCEDLA STLKMNQSLL ELDLGLNDLG DSGVLLLCEG LSHPDCKLQT LRLGICRLGS VACVGIASVL QVNTCLQELD LSFNDLGDRG LQLLGEGLRH QTCRLQKLWL DNCGLTSKAC EDLSSILGIS QTLHELYLTN NALGDTGVCL LCKRLRHPGC KLRVLWLFGM DLNKKTHRRM AALRVTKPYL DIGC
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: NLRP12 (NLR Family, Pyrin Domain Containing 12 (NLRP12))

Alternative Name: Nlrp12 (NLRP12 Products)

Synonyms: Nalp12 Protein, NALP12 Protein, CLR19.3 Protein, FCAS2 Protein, PAN6 Protein, PYPAF7 Protein, RNO Protein, RNO2 Protein, NLR family, pyrin domain containing 12 Protein, NLR family pyrin domain containing 12 Protein, NACHT, LRR and PYD domains-containing protein 3 Protein, Nlrp12 Protein, NLRP12 Protein, LOC100018508 Protein

Background: NACHT, LRR and PYD domains-containing protein 12 (Monarch-1) (PYRIN-containing APAF1-like protein 7) (PYPAF7),FUNCTION: Plays an essential role as an potent mitigator of inflammation (PubMed:26521018, PubMed:30559449). Primarily expressed in dendritic cells and macrophages, inhibits both canonical and non-canonical NF-kappa-B and ERK activation pathways (PubMed:30559449). Functions as a negative regulator of NOD2 by targeting it to degradation via the proteasome pathway (PubMed:30559449). In turn, promotes bacterial tolerance (PubMed:30559449). Inhibits also the RIGI-mediated immune signaling against RNA viruses by reducing the E3 ubiquitin ligase TRIM25-mediated 'Lys-63'-linked RIGI activation but enhancing the E3 ubiquitin ligase RNF125-mediated 'Lys-48'-linked RIGI degradation (By similarity). Acts also as a negative regulator of inflammatory response to mitigate obesity and obesity-associated diseases in adipose tissue (PubMed:30212649). {ECO:0000250|UniProtKB:P59046, ECO:0000269|PubMed:26521018, ECO:0000269|PubMed:30212649, ECO:0000269|PubMed:30559449}.

Molecular Weight: 119.3 kDa

UniProt: E9Q5R7

Pathways: Positive Regulation of Endopeptidase Activity, Inflammasome