FOXM1 Protein (AA 1-760) (His tag)
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- Target See all FOXM1 Proteins
- FOXM1 (Forkhead Box M1 (FOXM1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-760
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Origin
- Mouse
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Source
- Insect Cells
- Purification tag / Conjugate
- This FOXM1 protein is labelled with His tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- Sequence
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MRTSPRRPLI LKRRRLPLPV QNAPSETSEE EAKRSPAQPE PAPAQASQEV AESSSCKFPA GIKIINHPTT PNTQVVAIPS NADIQSIITA LTAKGKESGT SGPNRFILIS SGGPSSHPSQ PQAHSSRDSK RAEVITETLG PKPAAKGVPV PKPPGAPPRQ RQESYAGGEA AGCTLDNSLT NIQWLGKMSS DGLGPCSVKQ ELEEKENCHL EQNRVKVEEP SGVSTSWQDS VSERPPYSYM AMIQFAINST ERKRMTLKDI YTWIEDHFPY FKHIAKPGWK NSIRHNLSLH DMFVRETSAN GKVSFWTIHP SANRHLTLDQ VFKPLEPGSP QSPEHLESQQ KRPNPELHRN VTIKTEIPLG ARRKMKPLLP RVSSYLEPIQ FPVNQSLVLQ PSVKVPFRLA ASLMSSELAR HSKRVRIAPK VLLSSEGIAP LPATEPPKEE KPLLGGEGLL PLLPIQSIKE EEMQPEEDIA HLERPIKVES PPLEEWPSPC ASLKEELSNS WEDSSCSPTP KPKKSYCGLK SPTRCVSEML VTKRREKREV SRSRRKQHLQ PPCLDEPDLF FPEDSSTFRP AVELLAESSE PAPHLSCPQE EGGPFKTPIK ETLPVSSTPS KSVLSRDPES WRLTPPAKVG GLDFSPVRTP QGAFGLLPDS LGLMELNTTP LKSGPLFDSP RELLNSEPFD LASDPFGSPP PPHVEGPKPG SPELQIPSLS ANRSLTEGLV LDTMNDSLSK ILLDISFPGL EEDPLGPDNI NWSQFIPELR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Foxm1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product FOXM1 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- FOXM1 (Forkhead Box M1 (FOXM1))
- Alternative Name
- Foxm1 (FOXM1 Products)
- Synonyms
- FKHL16 Protein, FOXM1B Protein, HFH-11 Protein, HFH11 Protein, HNF-3 Protein, INS-1 Protein, MPHOSPH2 Protein, MPP-2 Protein, MPP2 Protein, PIG29 Protein, TGT3 Protein, TRIDENT Protein, FOXM1 Protein, AA408308 Protein, AW554517 Protein, BB238854 Protein, Fkh16 Protein, Foxm1b Protein, HFH-11B Protein, Mpm2 Protein, WIN Protein, Trident Protein, fkhl16 Protein, foxm1l Protein, hfh-11 Protein, mpp2 Protein, zgc:63854 Protein, forkhead box M1 Protein, forkhead box M1 S homeolog Protein, FOXM1 Protein, Foxm1 Protein, foxm1 Protein, foxm1.S Protein
- Background
- Transcriptional factor regulating the expression of cell cycle genes essential for DNA replication and mitosis. Plays a role in the control of cell proliferation. Plays also a role in DNA breaks repair participating in the DNA damage checkpoint response. {ECO:0000269|PubMed:17101782}.
- Molecular Weight
- 84.6 kDa Including tag.
- UniProt
- O08696
- Pathways
- Positive Regulation of Response to DNA Damage Stimulus
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