AKAP1 Protein (AA 30-857) (His tag)

Catalog No. ABIN3131293

Product Details

Target: AKAP1 (A Kinase (PRKA) Anchor Protein 1 (AKAP1))

Protein Type: Recombinant

Protein Characteristics: AA 30-857

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This AKAP1 protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: KDRLSSSDKQ VETLKVGPAI KDRRLSEEAC PGVLSVAPTV TQPPGREEQR CVDKPSTEPL ALPRTRQVRR RSESSGNLPS VADTRSQPGP CRDEIAKVEL SLMGDKAKSI PLGCPLLPKD ASFPYEAVER CKQESALGKT PGRGWPSPYA ASGEKARETG GTEGTGDAVL GENVSEEGLL SQECVSEVEK SEFPILAPGG GEGEEVSHGP PQVAELLKKE EYIVGKLPSS FVEPVHSEPV KDEDALEPQV KGSSNTSDRD LAGELDKDET VPENDQIKQA AFQLISQVIL EATEEFRATT VGKTVAQVHP TSATQPKGKE ESCVPASQET SLGQDTSDPA STRTGATASP SAEALPPKTY VSCLSSPLSG PTKDQKPKNS AHHISLAPCP PPVTPQRQSL EGASNPRGDD NFVACMANNS QSVLSVSSLG QCSDPVSTSG LEDSCTETIS SSGDKAMTPP LPVSTQPFSN GVLKEELSDL GTEDGWTMDT EADHSGGSDG NSMDSVDSCC GLTKPDSPQS VQAGSNPKKV DLIIWEIEVP KHLVGRLIGK QGRYVSFLKQ TSGAKIYIST LPYTQNIQIC HIEGSQHHVD KALNLIGKKF KELNLTNIYA PPLPSLALPS LPMTSWLMLP DGITVEVIVV NQVNAGHLFV QQHTHPTFHA LRSLDQQMYL CYSQPGIPTL PTPVEITVIC AAPGADGAWW RAQVVASYEE TNEVEIRYVD YGGYKRVKVD VLRQIRSDFV TLPFQGAEVL LDSVVPLSDD DHFSPEADAA MSEMTGNTAL LAQVTSYSAT GLPLIQLWSV VGDEVVLINR SLVERGLAQW VDSYYASL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Akap1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: AKAP1 (A Kinase (PRKA) Anchor Protein 1 (AKAP1))

Alternative Name: Akap1 (AKAP1 Products)

Synonyms: AKAP1 Protein, akap1 Protein, fa08b04 Protein, wu:fa08b04 Protein, AKAP Protein, AKAP121 Protein, AKAP149 Protein, AKAP84 Protein, D-AKAP1 Protein, PPP1R43 Protein, PRKA1 Protein, SAKAP84 Protein, TDRD17 Protein, Akap Protein, C76494 Protein, C81186 Protein, DAKAP1 Protein, S-AKAP84 Protein, Akap84 Protein, A-kinase anchoring protein 1 Protein, A kinase (PRKA) anchor protein 1b Protein, A kinase (PRKA) anchor protein 1 Protein, AKAP1 Protein, akap1b Protein, akap1 Protein, Akap1 Protein

Background: Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A. Anchors them to the cytoplasmic face of the mitochondrial outer membrane or allows them to reside in the endoplasmic reticulum. Does not contain the classic KDEL endoplasmic reticulum-targeting sequence. This explains how it is able to switch its localization, either being in the endoplasmic reticulum or in the mitochondria depending on which N-terminal part begins the isoform. The longest N-terminal part only present in isoform 2 and isoform 4 acts as a suppressor of mitochondrial targeting and as an activator of recessive endoplasmic reticulum targeting motif.

Molecular Weight: 89.7 kDa Including tag.

UniProt: O08715

Pathways: Synaptic Membrane