NCOA3 Protein (AA 2-1398) (His tag)

Catalog No. ABIN3131361

Product Details

Target: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Protein Type: Recombinant

Protein Characteristics: AA 2-1398

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This NCOA3 protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: SGLGESSLDP LAAESRKRKL PCDAPGQGLV YSGEKWRREQ ESKYIEELAE LISANLSDID NFNVKPDKCA ILKETVRQIR QIKEQGKTIS SDDDVQKADV SSTGQGVIDK DSLGPLLLQA LDGFLFVVNR DGNIVFVSEN VTQYLQYKQE DLVNTSVYSI LHEPRRKDFL NTYQNPQLME FLGLMRTRDK KAPYILIVRM LMKTHDILED VNASPETRQR YETMQCFALS QPRAMLEEGE DLQCCMICVA RRVTAPFPSS PESFITRHDL SGKVVNIDTN SLRSSMRPGF EDIIRRCIQR FFSLNDGQSW SQKRHYQEAY VHGHAETPVY RFSLADGTIV SAQTKSKLFR NPVTNDRHGF ISTHFLQREQ NGYRPNPIPQ DKGIRPPAAG CGVSMSPNQN VQMMGSRTYG VPDPSNTGQM GGARYGASSS VASLTPGQSL QSPSSYQNSS YGLSMSSPPH GSPGLGPNQQ NIMISPRNRG SPKMASHQFS PAAGAHSPMG PSGNTGSHSF SSSSLSALQA ISEGVGTSLL STLSSPGPKL DNSPNMNISQ PSKVSGQDSK SPLGLYCEQN PVESSVCQSN SRDPQVKKES KESSGEVSET PRGPLESKGH KKLLQLLTCS SDDRGHSSLT NSPLDPNCKD SSVSVTSPSG VSSSTSGTVS STSNVHGSLL QEKHRILHKL LQNGNSPAEV AKITAEATGK DTSSTASCGE GTTRQEQLSP KKKENNALLR YLLDRDDPSD VLAKELQPQA DSGDSKLSQC SCSTNPSSGQ EKDPKIKTET NDEVSGDLDN LDAILGDLTS SDFYNNPTNG GHPGAKQQMF AGPSSLGLRS PQPVQSVRPP YNRAVSLDSP VSVGSGPPVK NVSAFPGLPK QPILAGNPRM MDSQENYGAN MGPNRNVPVN PTSSPGDWGL ANSRASRMEP LASSPLGRTG ADYSATLPRP AMGGSVPTLP LRSNRLPGAR PSLQQQQQQQ QQQQQQQQQQ QQQQQQMLQM RTGEIPMGMG VNPYSPAVQS NQPGSWPEGM LSMEQGPHGS QNRPLLRNSL DDLLGPPSNA EGQSDERALL DQLHTFLSNT DATGLEEIDR ALGIPELVNQ GQALESKQDV FQGQEAAVMM DQKAALYGQT YPAQGPPLQG GFNLQGQSPS FNSMMGQISQ QGSFPLQGMH PRAGLVRPRT NTPKQLRMQL QQRLQGQQFL NQSRQALEMK MENPAGTAVM RPMMPQAFFN AQMAAQQKRE LMSHHLQQQR MAMMMSQPQP QAFSPPPNVT ASPSMDGVLA GSAMPQAPPQ QFPYPANYGM GQPPEPAFGR GSSPPSAMMS SRMGPSQNAM VQHPQPTPMY QPSDMKGWPS GNLARNGSFP QQQFAPQGNP AAYNMVHMNS SGGHLGQMAM TPMPMSGMPM GPDQKYC
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Ncoa3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Alternative Name: Ncoa3 (NCOA3 Products)

Synonyms: NCOA3 Protein, ACTR Protein, AIB-1 Protein, AIB1 Protein, CAGH16 Protein, CTG26 Protein, KAT13B Protein, RAC3 Protein, SRC-3 Protein, SRC3 Protein, TNRC14 Protein, TNRC16 Protein, TRAM-1 Protein, bHLHe42 Protein, pCIP Protein, 2010305B15Rik Protein, AW321064 Protein, Actr Protein, Aib1 Protein, Rac3 Protein, Src3 Protein, Tram-1 Protein, Tram1 Protein, p/Cip Protein, pCip Protein, actr Protein, aib-1 Protein, aib1 Protein, cagh16 Protein, ctg26 Protein, kat13b Protein, pcip Protein, rac3 Protein, src-3 Protein, src3 Protein, tnrc14 Protein, tnrc16 Protein, tram-1 Protein, xSRC-3 Protein, nuclear receptor coactivator 3 Protein, nuclear receptor coactivator 3 S homeolog Protein, NCOA3 Protein, Ncoa3 Protein, ncoa3.S Protein

Background: Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, probably via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit (By similarity). {ECO:0000250, ECO:0000269|PubMed:10823921}.

Molecular Weight: 152.4 kDa Including tag.

UniProt: O09000

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha