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MRPS12 Protein (AA 30-139) (His tag)

Crystallography grade MRPS12 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3131652
  • Target See all MRPS12 Proteins
    MRPS12 (Mitochondrial Ribosomal Protein S12 (MRPS12))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 30-139
    Origin
    • 2
    • 1
    • 1
    Mouse
    Source
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This MRPS12 protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    MATLNQMHRL GPRKEPPKRL GPTEGRPQLK GVVLRTFIRK PKKPNSANRK CCRVRLSTGK EAVCFIPGEG HTLQEHHVVL VEGGRTQDLP GVKLKVVRGK YDCGHVQKKK
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Mrps12 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Grade
    Crystallography grade
    Top Product
    Discover our top product MRPS12 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    MRPS12 (Mitochondrial Ribosomal Protein S12 (MRPS12))
    Alternative Name
    Mrps12 (MRPS12 Products)
    Synonyms
    MPR-S12 Protein, MT-RPS12 Protein, RPMS12 Protein, RPS12 Protein, RPSM12 Protein, AI327385 Protein, Rpms12 Protein, rps12 Protein, CG7925 Protein, Dmel\\CG7925 Protein, EG:BACH59J11.1 Protein, MRP-S12 Protein, MRPS12 Protein, S12 Protein, Tko Protein, l(1)3Ab Protein, l(1)tko Protein, mRpS12 Protein, mt-rps12 Protein, MGC64304 Protein, GB10531 Protein, mitochondrial ribosomal protein S12 Protein, technical knockout Protein, mitochondrial ribosomal protein S12 L homeolog Protein, 40S ribosomal protein S12, mitochondrial Protein, putative mitochondrial 37S ribosomal protein MRPS12 Protein, fragile site, aphidicolin type, common, fra(6)(p22.2) Protein, MRPS12 Protein, Mrps12 Protein, tko Protein, mrps12.L Protein, LOC409723 Protein, mRpS12 Protein, mrps12 Protein, CAALFM_C106070WA Protein, mrpS12 Protein, FRA6C Protein
    Molecular Weight
    13.3 kDa Including tag.
    UniProt
    O35680
    Pathways
    Sensory Perception of Sound
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