Neuroserpin Protein (AA 17-410) (His tag)

Catalog No. ABIN3131657

Product Details

Target: Neuroserpin (SERPINI1) (serpin Peptidase Inhibitor, Clade I (neuroserpin), Member 1 (SERPINI1))

Protein Type: Recombinant

Protein Characteristics: AA 17-410

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Neuroserpin protein is labelled with His tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)

Sequence: TGATFPDETI TEWSVNMYNH LRGTGEDENI LFSPLSIALA MGMMELGAQG STRKEIRHSM GYEGLKGGEE FSFLRDFSNM ASAEENQYVM KLANSLFVQN GFHVNEEFLQ MLKMYFNAEV NHVDFSQNVA VANSINKWVE NYTNSLLKDL VSPEDFDGVT NLALINAVYF KGNWKSQFRP ENTRTFSFTK DDESEVQIPM MYQQGEFYYG EFSDGSNEAG GIYQVLEIPY EGDEISMMLA LSRQEVPLAT LEPLLKAQLI EEWANSVKKQ KVEVYLPRFT VEQEIDLKDI LKALGVTEIF IKDANLTAMS DKKELFLSKA VHKSCIEVNE EGSEAAAASG MIAISRMAVL YPQVIVDHPF LYLIRNRKSG IILFMGRVMN PETMNTSGHD FEEL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Serpini1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Neuroserpin (SERPINI1) (serpin Peptidase Inhibitor, Clade I (neuroserpin), Member 1 (SERPINI1))

Alternative Name: Serpini1 (SERPINI1 Products)

Synonyms: PI12 Protein, neuroserpin Protein, AI837402 Protein, Ns Protein, PI-12 Protein, Spi17 Protein, CG9453 Protein, Dmel\\CG9453 Protein, Serp2 Protein, Sp4 Protein, Spn4 Protein, Spn4A Protein, dSerp2 Protein, sp4 Protein, spn4 Protein, raPIT5a Protein, SERPINI1 Protein, pi12 Protein, serpini1l Protein, si:ch211-167c22.4 Protein, serpin family I member 1 Protein, serine (or cysteine) peptidase inhibitor, clade I, member 1 Protein, Serpin 42Da Protein, serpin peptidase inhibitor, clade I (neuroserpin), member 1 Protein, serpin family I member 1 L homeolog Protein, SERPINI1 Protein, Serpini1 Protein, Spn42Da Protein, serpini1 Protein, serpini1.L Protein

Background: Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator.

Molecular Weight: 45.5 kDa Including tag.

UniProt: O35684

Pathways: Regulation of Hormone Metabolic Process