SLK Protein (AA 1-1233) (Strep Tag)

Catalog No. ABIN3131834

Product Details

Target: SLK (STE20-Like Kinase (SLK))

Protein Type: Recombinant

Protein Characteristics: AA 1-1233

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This SLK protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MSFFNFRKIF KLGSEKKKKQ YEHVKRDLNP EEFWEIIGEL GDGAFGKVYK AQNKETNVLA AAKVIDTKSE EELEDYMVEI DILASCDHPN IVKLLDAFYY ENNLWILIEF CAGGAVDAVM LELERPLTES QIQVVCKQTL EALNYLHDNK IIHRDLKAGN ILFTLDGDIK LADFGVSAKN TRTIQRRDSF IGTPYWMAPE VVMCETSKDR PYDYKADVWS LGITLIEMAE IEPPHHELNP MRVLLKIAKS EPPTLAQPSK WSSNFKDFLR KCLEKNVDAR WTTSQLLQHP FVTVDSNKPV RELIAEAKAE VTEEVEDGKE EDEEEEAENA LPIPANKRAS SDLSIASSEE DKLSQNACIL ESVSERTEQS TSEDKFSNKI LNEKPTTDGP EKAVDEHASD VNLETGAELN DQTVGIHENG REKKRPKLEN LPDTQDQQTV DVNSVSEENE NNRVTLETNT DCLKPEEDRN KENQETLESK LIQSEEINDT HIQTMDLVSQ ETGEKEADFQ AVDNEVGLTK EETQEKLGKD GTAQKVITSD RSSEVGTDEA LDDTQKAAEL SKAAQSGEGD EALAPTQTLA EKPTEGPEAG GAEEEPPGGE RVEDKQPEQQ PAVCEAEGQL TSTSETTRAT LEQPETDEVE QVSESNSIEE LERLVVTGAE ARALGSEGEA AATEVDLERK ENAQKVPVKA ESQAPAASQP SEPHPVLIPS ININSETTEN KEEMGALPKP ETILPPEPEH EKGNDTDSGT GSTVENSSGD LNLSISSFLS KAKDSGSVSL QETRRQKKTL KKTRKFIVDG VEVSVTTSKI VTDSDSKTEE LRFLRRQELR ELRLLQKEEQ RAQQQLNGKL QQQREQIFRR FEQEMLSKKR QYDQEIENLE KQQKQTIERL EQEHTNRLRD EAKRIKGEQE KELSKFQNVL KNRKKEVMNE VEKAPRELRR ELTKRRKEEL AQSQHAQEQE FVQKQQQELD GSLKKIIQQQ KAELANIERE CLNNKQQLMR AREAAIWELE ERHLQEKHQL LKQQLKDQYF MQRHQLLKRH EKETEQMQRY NQRLIEELKN RQTQERARLP KIQRSEAKTR MAMFKKSLRI NSTATPDQDR EKIKQFAAQE EKRQKNERMA QHQKHESQMR DLQLQCEANV RELHQLQNEK CHLLVEHETQ KLKELDEEHS QELKEWREKL RPRKKTLEEE FARKLQEQEV FFKMTGESEC LNPSAQSRIS KFYPIPTLHS TGS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SLK (STE20-Like Kinase (SLK))

Alternative Name: Slk (SLK Products)

Synonyms: BcDNA:LD05623 Protein, CG40293 Protein, Dmel\\CG40293 Protein, LD05623 Protein, LOSK Protein, STK2 Protein, bA16H23.1 Protein, se20-9 Protein, 9A2 Protein, AV021402 Protein, AW411554 Protein, Etk4 Protein, SMAK Protein, Stk2 Protein, mKIAA0204 Protein, mSLK Protein, STE20 like kinase Protein, Ste20-like kinase Protein, STE20-like kinase Protein, SLK Protein, Stlk Protein, Slk Protein

Background: STE20-like serine/threonine-protein kinase (STE20-like kinase) (mSLK) (EC 2.7.11.1) (Etk4) (STE20-related kinase SMAK) (STE20-related serine/threonine-protein kinase) (STE20-related kinase) (Serine/threonine-protein kinase 2),FUNCTION: Mediates apoptosis and actin stress fiber dissolution. {ECO:0000269|PubMed:10611247}.

Molecular Weight: 141.5 kDa

UniProt: O54988