PIP4K2A Protein (AA 2-405) (His tag)

Catalog No. ABIN3131930

Product Details

Target: PIP4K2A (Phosphatidylinositol-5-Phosphate 4-Kinase, Type II, alpha (PIP4K2A))

Protein Type: Recombinant

Protein Characteristics: AA 2-405

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PIP4K2A protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequence: ATPGNLGSSV LASKTKTKKK HFVAQKVKLF RASDPLLSVL MWGVNHSINE LSHVQIPVML MPDDFKAYSK IKVDNHLFNK ENMPSHFKFK EYCPMVFRNL RERFGIDDQD FQNSLTRSAP LPNDSQARSG ARFHTSYDKR YVIKTITSED VAEMHNILKK YHQYIVECHG VTLLPQFLGM YRLNVDGVEI YVIVTRNVFS HRLSVYRKYD LKGSTVAREA SDKEKAKELP TLKDNDFINE GQKIYIDDNN KKIFLEKLKK DVEFLAQLKL MDYSLLVGIH DVERAEQEEV ECEENDGEEE GESDSTHPIG TPPDSPGNTL NSSPPLAPGE FDPNIDVYAI KCHENAPRKE VYFMAIIDIL THYDAKKKAA HAAKTVKHGA GAEISTVNPE QYSKRFLDFI GHIL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Pip4k2a Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PIP4K2A (Phosphatidylinositol-5-Phosphate 4-Kinase, Type II, alpha (PIP4K2A))

Alternative Name: Pip4k2a (PIP4K2A Products)

Synonyms: PI5P4KA Protein, PIP5K2A Protein, PIP5KII-alpha Protein, PIP5KIIA Protein, PIPK Protein, AW742916 Protein, Pip5k2a Protein, pipk Protein, pi5p4ka Protein, pip5k2a Protein, pip5kiia Protein, pip4k2a Protein, zgc:194746 Protein, zgc:194777 Protein, phosphatidylinositol-5-phosphate 4-kinase type 2 alpha Protein, phosphatidylinositol-5-phosphate 4-kinase, type II, alpha Protein, phosphatidylinositol-5-phosphate 4-kinase, type II, alpha S homeolog Protein, phosphatidylinositol-5-phosphate 4-kinase, type II, alpha a Protein, PIP4K2A Protein, Pip4k2a Protein, pip4k2a.S Protein, pip4k2a Protein, pip4k2aa Protein

Background: Catalyzes the phosphorylation of phosphatidylinositol 5-phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation. May negatively regulate insulin-stimulated glucose uptake by lowering the levels of PtdIns5P (By similarity). May be involved in thrombopoiesis and the terminal maturation of megakaryocytes and regulation of their size. {ECO:0000250, ECO:0000269|PubMed:16434494}.

Molecular Weight: 47.0 kDa Including tag.

UniProt: O70172

Pathways: Inositol Metabolic Process