PLIN4 Protein (AA 1-1403) (Strep Tag)

Catalog No. ABIN3132124

Product Details

Target: PLIN4 (Perilipin 4 (PLIN4))

Protein Type: Recombinant

Protein Characteristics: AA 1-1403

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This PLIN4 protein is labelled with Strep Tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Sequence: MSASGDGTRV PPKSKGKTLS SFFGSLPGFS SARNLVSHTH SSTSTKDLQT ATDPSGTPAP SSKVSTNSQM AGDAAGLLQP SEQTAGDKDM GSFSVTSSED AFSGVFGIMD AAKGMVQGGL GATQSALVGT KEAVSGGVMG AVGVAKGLVK GGLDTSKNVL TNTKDTVTTG VMGAANMAKG TVQTGLDTTK SVVMGTKDTV ATGLAGAVNV AKGTIQGGLD TTKSVVMGTK DTVTTGLTGA VNVAKGVVQG GLDTTKSVVM GTKDTVTTGL TGAMNVAKGT AQMGIDTSKT VLTGTKDTVC AGATGAINVA KGAAQGGLDT TKSVLIGTKD TVTTGLTGAV NVAKGAVQGG LDTTKSVVMG TKDTVTTGLT GAMNVAKGTA QMGLGTSKTV LTGTKDTVCA GLTGAINVAK GAAQGGLDTT KSVLMGTKDT VTTGLTGAVN VAKGTIQGGL DTTKSVVMGT KDTVTTGLTG AVNVAKGTIQ GGLDTTKSVV MGTKDTVTTG LTGAVNVAKG AAQGGLDTTK SVVMGTKDTV TTGLTGAMNV AKGTAQMGLG TSKTVLTGTK DTVCAGLTGA INVAKGAAQG GLDTTKSVLM GTKDTVTTGL TGAVNVAKGT IQGGLDTTKS VVMGTKDTVT TGLTGAVNVA KGAVQGGLDT TKSVVMGTKD TVTTGLTGAL NVAKGTAQMG IDTSKTVLIG TKDTVCAGAT GAINMAKGAA QGGLDTTKSV LMGTKDTVTT GLTGAINVAK GSAQGGLDTT KSVLIGTKDT VTTGLTGALN VAKGTVQTGL DTSQRVLTGT KDNVYAGVTG AVNVAKGTIQ GGLDTTKSVV MGTKDTVTTG LTGAVNVAKG AVQGGLDTTK SVVMGTKDTV TTGLTGAMNV AKGTAQMGID TSKTVLTGTK DTVCAGLTGA INVAKGATQG GLDTTKSVLM GTKDTVTTGL TGAINVAKGA AQGGLDTTKS VLLGTKDTVT TGLTGAANVA KETVQMGLDT SKNILMDTKD SICAGATGAI TVVKGAAQGG LDTSNAALTG TMDTAKGTVQ TSLDTSKHML IGMKDTVCAG VTSAMNMAKG IHKNTDTTRD TQSSVLAHSG NVATNAIHTG VHTVPSSLSG SHSIICHEPS IYRATNHGVG QAILTSTESL CCETSSFSDK YGLGHVTEPR ADTKTLVSGM ASSACAATRS VEECGQLAAT GFAALPDELK GLGDIFQPMT TEEQAQLAVS ESGPRVLSAD RGSYYIRLGD LAPSFRQRAF EHALSHIQHN QFQARAALAQ LQEAFQMTDM TMEAACGKLC SDQSLNTMVE AVGSHEMRAS VAQDRLCTLA HQLHAAYSSL VTSLQGLPEV QQQAGQARHS LCKLYGLVSS EAGSELQTEQ LAQSSAGVVE AWQGLEVLLE KLQQNPPLSW LVGPFTSMPC GQL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PLIN4 (Perilipin 4 (PLIN4))

Alternative Name: Plin4 (PLIN4 Products)

Synonyms: PLIN4 Protein, KIAA1881 Protein, S3-12 Protein, mKIAA1881 Protein, perilipin 4 Protein, perilipin-4 Protein, PLIN4 Protein, LOC510990 Protein, Plin4 Protein

Background: Perilipin-4 (Adipocyte protein S3-12),FUNCTION: May play a role in triacylglycerol packaging into adipocytes. May function as a coat protein involved in the biogenesis of lipid droplets. {ECO:0000269|PubMed:12840023, ECO:0000269|PubMed:15731108}.

Molecular Weight: 139.4 kDa

UniProt: O88492