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CYP1A1 Protein (AA 1-524) (His tag)

Crystallography grade CYP1A1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Catalog No. ABIN3132333
  • Target See all CYP1A1 Proteins
    CYP1A1 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-524
    Origin
    • 4
    • 4
    • 2
    • 1
    • 1
    Mouse
    Source
    • 8
    • 1
    • 1
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This CYP1A1 protein is labelled with His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequence
    MPSMYGLPAF VSATELLLAV TVFCLGFWVV RATRTWVPKG LKTPPGPWGL PFIGHMLTVG KNPHLSLTRL SQQYGDVLQI RIGSTPVVVL SGLNTIKQAL VRQGDDFKGR PDLYSFTLIT NGKSMTFNPD SGPVWAARRR LAQNALKSFS IASDPTSASS CYLEEHVSKE ANYLVSKLQK VMAEVGHFDP YKYLVVSVAN VICAICFGQR YDHDDQELLS IVNLSNEFGE VTGSGYPADF IPVLRYLPNS SLDAFKDLND KFYSFMKKLI KEHYRTFEKG HIRDITDSLI EHCQDRKLDE NANVQLSDDK VITIVLDLFG AGFDTVTTAI SWSLMYLVTN PRVQRKIQEE LDTVIGRDRQ PRLSDRPQLP YLEAFILETF RHSSFVPFTI PHSTTRDTSL NGFYIPKGCC VFVNQWQVNH DRELWGDPNE FRPERFLTPS GTLDKRLSEK VTLFGLGKRK CIGETIGRSE VFLFLAILLQ QIEFKVSPGE KVDMTPTYGL TLKHARCEHF QVQMRSSGPQ HLQA
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Cyp1a1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product CYP1A1 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    CYP1A1 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1))
    Alternative Name
    Cyp1a1 (CYP1A1 Products)
    Synonyms
    AHH Protein, AHRR Protein, CP11 Protein, CYP1 Protein, P1-450 Protein, P450-C Protein, P450DX Protein, Cyp1a2 Protein, P450-1 Protein, cyp1a1 Protein, CYP1A1 Protein, CYPIA1 Protein, Cyp45c Protein, Cypc45c Protein, P-450MC Protein, wu:fb63b04 Protein, zfCYP1A Protein, zgc:109747 Protein, ahh Protein, ahrr Protein, cp11 Protein, cyp1 Protein, cyp1a Protein, p1-450 Protein, p450-c Protein, p450dx Protein, cytochrome P450 family 1 subfamily A member 1 Protein, cytochrome P450 1A1 Protein, cytochrome P450, family 1, subfamily a, polypeptide 1 Protein, cytochrome P450, family 1, subfamily A, polypeptide 1 Protein, cytochrome P450 family 1 subfamily D polypeptide 1 Protein, cytochrome P4501A1 Protein, polycyclic hydrocarbon-inducible cytochrome P450c Protein, cytochrome P450, family 1, subfamily A Protein, cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1 Protein, cytochrome P450 family 1 subfamily A member 1 L homeolog Protein, CYP1A1 Protein, CpipJ_CPIJ010542 Protein, Cyp1a1 Protein, cyp1d1 Protein, LOC100328613 Protein, cyp1a Protein, LOC102129994 Protein, cyp1a1.L Protein
    Background
    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
    Molecular Weight
    60.2 kDa Including tag.
    UniProt
    P00184
    Pathways
    Steroid Hormone Biosynthesis, Regulation of Lipid Metabolism by PPARalpha
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