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Surfeit 1 (SURF1) (AA 1-306) protein (rho-1D4 tag)

Details for Product No. ABIN3132711, Supplier: Log in to see
Protein Name
  • BcDNA:GH23574
  • CG9943
  • Dmel\\CG9943
  • GB14201
  • SURF1
  • im:6898613
  • zgc:158646
  • Ab1-205
  • SURF-1
  • 0610010F23Rik
  • Surf-1
  • SURFEIT 1
  • Surfeit 1
  • surfeit 1
  • surfeit gene 1
  • Surfeit locus 1 cytochrome c oxidase biogenesis protein
  • Surf1
  • SURF1
  • surf1
Protein Characteristics
AA 1-306
2
2
1
1
Origin
Mouse (Murine)
8
1
Source
Insect Cells
4
1
1
Protein Type
Recombinant
Purification tag / Conjugate
This SURF1 protein is labelled with rho-1D4 tag.
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
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Available images

Sequence MAAVMALAVL PRRMTRWSQW AYAGRAQFCA VRRSVFGFSV RSGMVCRPRR CCSSTAETAA AKAEDDSFLQ WFLLLIPATA FGLGTWQVQR RKWKLKLIAE LESRVMAEPI PLPADPMELK NLEYRPVKVR GHFDHSKELY IMPRTMVDPV REARDAGRLS STESGAHVVT PFHCSDLGVT ILVNRGFVPR KKVNPETRQK GQVLGEVDLV GIVRLTENRK PFVPENSPER NHWYYRDLEA MAKITGADPI FIDADFHSTA PGGPIGGQTR VTLRNEHMQY ILTWYGLCAA TSYLWFQKFV RRTPIM
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Characteristics
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Surf1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
Purification Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
  1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
  2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
  3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Purity >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterility 0.22 μm filtered
Endotoxin Level Protein is endotoxin-free.
Grade Crystallography grade
Background Probably involved in the biogenesis of the COX complex. {ECO:0000250}.
Molecular Weight 36.0 kDa Including tag.
UniProt P09925
Research Area Signaling, Organelles, Metabolism
Pathways Proton Transport, Ribonucleoside Biosynthetic Process
Application Notes In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Comment

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions For Research Use only
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -80 °C
Storage Comment Store at -80°C.
Expiry Date Unlimited (if stored properly)
Supplier Images
 image for Surfeit 1 (SURF1) (AA 1-306) protein (rho-1D4 tag) (ABIN3132711) „Crystallography Grade“ protein due to multi-step, protein-specific purification process