MSX1 Protein (AA 1-303) (Strep Tag)

Catalog No. ABIN3132933

Product Details

Target: MSX1 (Msh Homeobox 1 (MSX1))

Protein Type: Recombinant

Protein Characteristics: AA 1-303

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This MSX1 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MAPAAAMTSL PLGVKVEDSA FAKPAGGGVG QAPGAAAATA TAMGTDEEGA KPKVPASLLP FSVEALMADH RKPGAKESVL VASEGAQAAG GSVQHLGTRP GSLGAPDAPS SPRPLGHFSV GGLLKLPEDA LVKAESPEKL DRTPWMQSPR FSPPPARRLS PPACTLRKHK TNRKPRTPFT TAQLLALERK FRQKQYLSIA ERAEFSSSLS LTETQVKIWF QNRRAKAKRL QEAELEKLKM AAKPMLPPAA FGLSFPLGGP AAVAAAAGAS LYSASGPFQR AALPVAPVGL YTAHVGYSMY HLT
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: MSX1 (Msh Homeobox 1 (MSX1))

Alternative Name: Msx1 (MSX1 Products)

Synonyms: ECTD3 Protein, HOX7 Protein, HYD1 Protein, STHAG1 Protein, AA675338 Protein, AI324650 Protein, Hox-7 Protein, Hox7 Protein, Hox7.1 Protein, msh Protein, CHOX-7 Protein, GHOX-7 Protein, HOX-7 Protein, msh homeobox 1 Protein, MSX1 Protein, Msx1 Protein

Background: Homeobox protein MSX-1 (Homeobox protein Hox-7) (Hox-7.1) (Msh homeobox 1-like protein),FUNCTION: Acts as a transcriptional repressor (PubMed:7823952, PubMed:22629437, PubMed:23371388). Capable of transcription autoinactivation (PubMed:10215616). Binds to the consensus sequence 5'-C/GTAAT-3' in downstream activin regulatory elements (DARE) in the gene promoter, thereby repressing the transcription of CGA/alpha-GSU and GNRHR (PubMed:23371388). Represses transcription of myoblast differentiation factors (PubMed:16600910, PubMed:22629437). Binds to core enhancer regions in target gene promoters of myoblast differentiation factors with binding specificity facilitated by interaction with PIAS1 (PubMed:16600910). Recruits histone H3 methyltransferases such as EHMT2/G9a to gene promoter regions which leads to inhibition of myoblast differentiation via transcriptional repression of differentiation factors (PubMed:22629437). Regulates, in a stage-specific manner, a developmental program of gene expression in the fetal tooth bud that controls odontoblast differentiation and proliferation of dental mesenchymal cells (PubMed:7914451, PubMed:8898217, PubMed:24028588, PubMed:27713059, PubMed:29148101). At the bud stage, required for mesenchymal molar tooth bud development via facilitating reciprocal signaling between dental epithelial and mesenchymal cells (PubMed:8898217). May also regulate expression of Wnt antagonists such as DKK2 and SFPR2 in the developing tooth mesenchyme (PubMed:27713059). Required for BMP4 expression in dental mesenchyme cells (PubMed:8898217). Also, in response to BMP4, required for BMP4 expression in neighboring dental epithelial cells (PubMed:8898217). Required for maximal FGF4-induced expression of SDC1 in dental mesenchyme cells (PubMed:8898217). Also in response to SDC1, required for SDC1 expression in neighboring dental epithelial cells (PubMed:8898217). At the early bell stage, acts to drive proliferation of dental mesenchyme cells, however during the late bell stage acts as an homeostatic regulator of the cell cycle (PubMed:24028588, PubMed:29148101). Regulates proliferation and inhibits premature mesenchymal odontogenesis during the bell stage via inhibition of the Wnt signaling component CTNNB1 and subsequent repression of the odontoblast differentiation factors BMP2, BMP4, LEF1, ALPL and BGLAP/OCN (PubMed:24028588, PubMed:29148101). Additionally, required for correct development and fusion of the palatal shelves and embryonic mandibular formation (PubMed:7914451). Plays a role in embryonic bone formation of the middle ear, skull and nasal bones (PubMed:7914451). Required for correct formation and thickness of the nail plate (PubMed:11369996). May play a role in limb-pattern formation (PubMed:1677742). {ECO:0000250|UniProtKB:P28360, ECO:0000269|PubMed:10215616, ECO:0000269|PubMed:11369996, ECO:0000269|PubMed:16600910, ECO:0000269|PubMed:1677742, ECO:0000269|PubMed:22629437, ECO:0000269|PubMed:23371388, ECO:0000269|PubMed:24028588, ECO:0000269|PubMed:27713059, ECO:0000269|PubMed:29148101, ECO:0000269|PubMed:7823952, ECO:0000269|PubMed:7914451, ECO:0000269|PubMed:8898217}.

Molecular Weight: 31.7 kDa

UniProt: P13297

Pathways: Regulation of Muscle Cell Differentiation, Positive Regulation of Response to DNA Damage Stimulus