GPD1 Protein (AA 1-349) (Strep Tag)

Catalog No. ABIN3132955

Product Details

Target: GPD1 (Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1))

Protein Type: Recombinant

Protein Characteristics: AA 1-349

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This GPD1 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MAGKKVCIVG SGNWGSAIAK IVGSNAGRLA HFDPRVTMWV FEEDIGGRKL TEIINTQHEN VKYLPGHKLP PNVVAIPDVV QAATGADILV FVVPHQFIGK ICDQLKGHLK ANTIGISLIK GVDEGPNGLK LISEVIGERL GIPMSVLMGA NIASEVAEEK FCETTIGCKD PAQGQLLKDL MQTPNFRITV VQEVDTVEIC GALKNIVAVG AGFCDGLGFG DNTKAAVIRL GLMEMIAFAK LFCSGTVSSA TFLESCGVAD LITTCYGGRN RKVAEAFART GKSIEQLEKE MLNGQKLQGP QTARELHSIL QHKGLVDKFP LFTAVYKVCY EGQPVGEFIR CLQNHPEHM
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: GPD1 (Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1))

Alternative Name: Gpd1 (GPD1 Products)

Synonyms: GPDH Protein, Gpd3 Protein, GPD-C Protein, GPDH-C Protein, HTGTI Protein, CG9042 Protein, DROGPDHA Protein, DmG3PDH Protein, Dmel\\CG9042 Protein, G3PDH Protein, G3pdh Protein, GAPDH Protein, GPD Protein, GPDA Protein, GPDH-1 Protein, Gdh Protein, Gpd Protein, alpha-GPD Protein, alpha-GPDH Protein, alpha-GPDH-1 Protein, alpha-Gpdh Protein, alphaGPDH Protein, alphaGpd Protein, alphaGpdh Protein, alphaGpdh-1 Protein, gpdh Protein, gpdh-1 Protein, sn-Gpdh Protein, AI747587 Protein, Gdc-1 Protein, Gdc1 Protein, mKIAA4010 Protein, gpd1 Protein, wu:fc30a07 Protein, zgc:63859 Protein, MGC79676 Protein, GPD1 Protein, LG3P Protein, zgc:112197 Protein, Gpd1 Protein, gpd1h Protein, gpd1l Protein, wu:fc58b05 Protein, zgc:66051 Protein, zgc:85742 Protein, glycerol-3-phosphate dehydrogenase 1 Protein, Glycerol-3-phosphate dehydrogenase [NAD(+)] Protein, glycerol-3-phosphate dehydrogenase Protein, Glycerol 3 phosphate dehydrogenase Protein, glycerol-3-phosphate dehydrogenase 1 (soluble) Protein, glycerol-3-phosphate dehydrogenase 1 L homeolog Protein, glycerol-3-phosphate dehydrogenase 1c Protein, glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic Protein, glycerol-3-phosphate dehydrogenase 1a Protein, glycerol-3-phosphate dehydrogenase 1b Protein, Gpd1 Protein, gpdh-1 Protein, GPD1 Protein, Tb11.02.5280 Protein, Gpdh Protein, gpd1.L Protein, gpd1c Protein, gpd1 Protein, LOC101071719 Protein, gpd1a Protein, gpd1b Protein

Background: Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic (GPD-C) (GPDH-C) (EC 1.1.1.8),FUNCTION: Has glycerol-3-phosphate dehydrogenase activity. {ECO:0000250|UniProtKB:P21695}.

Molecular Weight: 37.6 kDa

UniProt: P13707