BLK Protein (AA 2-499) (His tag)

Catalog No. ABIN3133093

Product Details

Target: BLK (B Lymphoid Tyrosine Kinase (BLK))

Protein Type: Recombinant

Protein Characteristics: AA 2-499

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This BLK protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: GLLSSKRQVS EKGKGWSPVK IRTQDKAPPP LPPLVVFNHL APPSPNQDPD EEERFVVALF DYAAVNDRDL QVLKGEKLQV LRSTGDWWLA RSLVTGREGY VPSNFVAPVE TLEVEKWFFR TISRKDAERQ LLAPMNKAGS FLIRESESNK GAFSLSVKDI TTQGEVVKHY KIRSLDNGGY YISPRITFPT LQALVQHYSK KGDGLCQKLT LPCVNLAPKN LWAQDEWEIP RQSLKLVRKL GSGQFGEVWM GYYKNNMKVA IKTLKEGTMS PEAFLGEANV MKTLQHERLV RLYAVVTREP IYIVTEYMAR GCLLDFLKTD EGSRLSLPRL IDMSAQVAEG MAYIERMNSI HRDLRAANIL VSETLCCKIA DFGLARIIDS EYTAQEGAKF PIKWTAPEAI HFGVFTIKAD VWSFGVLLME IVTYGRVPYP GMSNPEVIRS LEHGYRMPCP ETCPPELYND IITECWRGRP EERPTFEFLQ SVLEDFYTAT EGQYELQP
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Blk Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: BLK (B Lymphoid Tyrosine Kinase (BLK))

Alternative Name: Blk (BLK Products)

Synonyms: bltk Protein, si:dkey-33i22.2 Protein, zgc:136231 Protein, MODY11 Protein, BLK proto-oncogene, Src family tyrosine kinase Protein, B lymphoid kinase Protein, blk Protein, BLK Protein, Blk Protein

Background: Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor. Specifically binds and phosphorylates CD79A at 'Tyr-188'and 'Tyr-199', as well as CD79B at 'Tyr-196' and 'Tyr-207'. Phosphorylates also the immunoglobulin G receptor FCGR2. With FYN and LYN, plays an essential role in pre-B-cell receptor (pre-BCR)-mediated NF-kappa-B activation. Contributes also to BTK activation by indirectly stimulating BTK intramolecular autophosphorylation. In pancreatic islets, acts as a modulator of beta-cells function through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose. {ECO:0000269|PubMed:12563261, ECO:0000269|PubMed:14662906, ECO:0000269|PubMed:2404338, ECO:0000269|PubMed:7565679, ECO:0000269|PubMed:7592958, ECO:0000269|PubMed:7608542, ECO:0000269|PubMed:7690139, ECO:0000269|PubMed:9177269, ECO:0000269|PubMed:9636152}.

Molecular Weight: 57.5 kDa Including tag.

UniProt: P16277

Pathways: Positive Regulation of Peptide Hormone Secretion, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling