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D Amino Acid Oxidase Protein (AA 1-345) (His tag)

Crystallography grade DAO Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Catalog No. ABIN3133207
  • Target See all D Amino Acid Oxidase (DAO) Proteins
    D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-345
    Origin
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Source
    • 10
    • 3
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This D Amino Acid Oxidase protein is labelled with His tag.
    Application
    SDS-PAGE (SDS), ELISA, Western Blotting (WB), Crystallization (Crys)
    Sequence
    MRVAVIGAGV IGLSTALCIH ERYHPTQPLH MKIYADRFTP FTTSDVAAGL WQPYLSDPSN PQEAEWSQQT FDYLLSCLHS PNAEKMGLAL ISGYNLFRDE VPDPFWKNAV LGFRKLTPSE MDLFPDYGYG WFNTSLLLEG KSYLPWLTER LTERGVKLIH RKVESLEEVA RGVDVIINCT GVWAGALQAD ASLQPGRGQI IQVEAPWIKH FILTHDPSLG IYNSPYIIPG SKTVTLGGIF QLGNWSGLNS VRDHNTIWKS CCKLEPTLKN ARIVGELTGF RPVRPQVRLE REWLRHGSSS AEVIHNYGHG GYGLTIHWGC AMEAANLFGK ILEEKKLSRL PPSHL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Dao Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product DAO Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))
    Alternative Name
    Dao (DAO Products)
    Synonyms
    DAAO Protein, DAMOX Protein, OXDA Protein, daao Protein, oxda Protein, damox Protein, DAO Protein, Dao1 Protein, DKFZp469N2232 Protein, AI987963 Protein, Dao-1 Protein, SC5F2A.23c Protein, wu:fb55b11 Protein, zgc:113922 Protein, ABP Protein, ABP1 Protein, DAO1 Protein, KAO Protein, MGC114783 Protein, Abp Protein, Abp1 Protein, D-amino acid oxidase Protein, D-amino-acid oxidase Protein, D-amino-acid oxidase, tandem duplicate 1 Protein, amine oxidase, copper containing 1 Protein, D-amino-acid oxidase L homeolog Protein, DAO Protein, Dao Protein, dao Protein, SCO6740 Protein, dao.1 Protein, AOC1 Protein, PTRG_02224 Protein, SJAG_02496 Protein, dao.L Protein, Aoc1 Protein
    Background
    Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.
    Molecular Weight
    39.6 kDa Including tag.
    UniProt
    P18894
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