EIF3A Protein (AA 2-1344) (His tag)

Catalog No. ABIN3133347

Product Details

Target: EIF3A (Eukaryotic Translation Initiation Factor 3 Subunit A (EIF3A))

Protein Type: Recombinant

Protein Characteristics: AA 2-1344

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This EIF3A protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: PAYFQRPENA LKRANEFLEV GKKQPALDVL YDVMKSKKHR TWQKIHEPIM LKYLELCVDL RKSHLAKEGL YQYKNICQQV NIKSLEDVVR AYLKLAEEKT EAAKEESQQM VLDIEDLDNI QTPESVLLSA VSGEDTQDRT DRLLLTPWVK FLWESYRQCL DLLRNNSRVE RLYHDIAQQA FKFCLQYTRK AEFRKLCDNL RMHLSQIQRH HNQSTAINLN NPESQSMHLE TRLVQLDSAI SMELWQEAFK AVEDIHGLFS LSKKPPKPQL MANYYNKVST VFWKSGNALF HASTLHRLYH LSREMRKNLT QEEMQRMSTR VLLATLSIPI TPERTDIARL LDMDGIIVEK QRRLATLLGL QAPPTRIGLI NDMVRFSVLQ YVVPEVKDLY NWLEVEFNPL KLCERVTKVL NWVREQPEKE PELQQYVPQL QNNTILRLLQ QVAQIYQSIE FSRLTSLVPF VDAFQLERAI VDAARHCDLQ VRIDHTSRTL SFGSDLNYAT REDAPVGPHL QSMPSEQIRN QLTAMSSVLA KALEVIRPAH ILQEKEEQHQ LAVNAYLKNS RKEHQRILAR RQTIEERKER LESLNIQREK EELEQREAEL QKVRKAEEER LRQEAKEREK ERILQEHEQI KKKTVRERLE QIKKTELGAK AFKDIDIEDL EELDPDFIMA KQVEQLEKEK KELQERLKNQ EKKIDYFERA KRLEEIPLIK SAYEEQRVKD MDLWEQQEEE RITTMQLERE KALEHKNRMS RMLEDRDLFV MRLKAARQSV YEEKLKQFEE RLAEERHSRL EDRKRQRKEE RKITYYREKE EEEQRRAEEQ MLKEREERER AERAKREEEL REYQERVKKL EEVERKKRQR ELEIEERERR REEERRLGDD PLSRKDSRWG DRDSEGTWRK GPEADSEWRR GPPEKEWRRE TRDDERPHRR DEDRLRRLGG DDEERESSLR PDDDRIPRRG LDDDRGPRRG PDEDRFSRRG TDDDRPSWRN ADDDRPPRRI GDDDRGSWRH TDDDRPPRRG LDDERGSWRT ADEDRGPRRG MDDDRGPRRG GADDERSSWR NADDDRGPRR GMDDDRGPRR GLDDDRGPWR NAAEDRISRR GADDDRGPWR NMDDDRVPRR GDDARPGPWR PFVKPGGWRE KEKAREESWG PPRESRPSEE REWDRDKEKD RDNQDREEND KDLERDRDRE RDGDREDRFR RPRDEGGWRR GPAEESSSWR DSSRRDDRDR EDRRRDRDDR RDLRDLRDRR DLRDDRDRRG PPLRSEREEA SSWRRTDDRK DDRTEERDPP RRVPPPALSR DREREREREG EKEKASWRAE KDRESLRRTK NETDEDGWTT VRR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Eif3a Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: EIF3A (Eukaryotic Translation Initiation Factor 3 Subunit A (EIF3A))

Alternative Name: Eif3a (EIF3A Products)

Synonyms: EIF3 Protein, EIF3S10 Protein, P167 Protein, TIF32 Protein, eIF3-p170 Protein, eIF3-theta Protein, p180 Protein, p185 Protein, EIF6 Protein, A830012B05Rik Protein, Csma Protein, Eif3 Protein, Eif3s10 Protein, mKIAA0139 Protein, ZH12 Protein, eif3s10 Protein, eIF3 p110 Protein, eIF3a Protein, eukaryotic translation initiation factor 3 subunit A Protein, eukaryotic translation initiation factor 3, subunit A Protein, eukaryotic translation initiation factor 3 subunit A S homeolog Protein, translation initiation factor eIF3a Protein, EIF3A Protein, Eif3a Protein, eif3a.S Protein, tif32 Protein

Background: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Molecular Weight: 162.8 kDa Including tag.

UniProt: P23116

Pathways: Ribonucleoprotein Complex Subunit Organization