ZFP36L2 Protein (AA 1-484) (Strep Tag)

Catalog No. ABIN3133387

Product Details

Target: ZFP36L2 (Zinc Finger Protein 36, C3H Type-Like 2 (ZFP36L2))

Protein Type: Recombinant

Protein Characteristics: AA 1-484

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This ZFP36L2 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MSTTLLSPFY DIDFLCKTEK SLANLNLNNM LDKKAVGTPV AAAPSSSFTP GFLRRHSASN LHALAHPVPS PGSCSPKFPG APNGGGSSCG PAGGGGLASY GQLKEPSGGS GTALVTKESK FRDRSFSENG ERSQHLLHLQ QQQKGGSGSQ INSTRYKTEL CRPFEESGTC KYGEKCQFAH GFHELRSLTR HPKYKTELCR TFHTIGFCPY GPRCHFIHNA DERRPAPSGG GGASGDLRAF GARDALHLGF AREPRPKLHH SLSFSGFPSG HHQPPGGLES PLLLDSPTSR TPPPPSSSAS SCSSSASSCS SASAASTPSG APTCCATAAA AALLYGPGGA EDLLSPGAPC ASCSSSGANN AFAFGPELSS LITPLAIQTH NFAAAAAAAY YRNQQQGLTG PAPPPAQPPA APAPPSPPFG FQLPRRLSES PVFDAPPSPP DSLSDRDSYL SGSLSSGSLS GSESPSLDPG RRLPIFSRLS ISDD
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ZFP36L2 (Zinc Finger Protein 36, C3H Type-Like 2 (ZFP36L2))

Alternative Name: Zfp36l2 (ZFP36L2 Products)

Synonyms: BRF2 Protein, ERF-2 Protein, ERF2 Protein, RNF162C Protein, TIS11D Protein, Brf2 Protein, Tis11d Protein, fi22c11 Protein, wu:fi22c11 Protein, zgc:76924 Protein, ZFP36L2 Protein, XC3H-3 Protein, XC3H-3b Protein, c3h-3 Protein, zfp36l2 Protein, brf2 Protein, erf-2 Protein, erf2 Protein, rnf162c Protein, tis11d Protein, cb198 Protein, cb480 Protein, fk89d11 Protein, id:ibd1247 Protein, sb:cb198 Protein, sb:cb480 Protein, wu:fb80h11 Protein, wu:fb92f07 Protein, wu:fk89d11 Protein, zfp36l1 Protein, zgc:153979 Protein, XC3H-2 Protein, berg36 Protein, brf1 Protein, c3h-2 Protein, cmg1 Protein, erf-1 Protein, erf1 Protein, rnf162b Protein, tis11b Protein, zfp36l1-a Protein, zfp36l1-b Protein, ZFP36 ring finger protein like 2 Protein, zinc finger protein 36, C3H type-like 2 Protein, ZFP36 ring finger protein-like 2 S homeolog Protein, ZFP36 ring finger protein-like 2 L homeolog Protein, ZFP36 ring finger protein-like 2 Protein, zinc finger protein 36, C3H type-like 1a Protein, ZFP36 ring finger protein-like 1 L homeolog Protein, ZFP36L2 Protein, Zfp36l2 Protein, zfp36l2 Protein, zfp36l2.S Protein, zfp36l2.L Protein, zfp36l1a Protein, zfp36l1.L Protein

Background: MRNA decay activator protein ZFP36L2 (Butyrate response factor 2) (TPA-induced sequence 11d) (Zinc finger protein 36, C3H1 type-like 2) (ZFP36-like 2),FUNCTION: Zinc-finger RNA-binding protein that destabilizes several cytoplasmic AU-rich element (ARE)-containing mRNA transcripts by promoting their poly(A) tail removal or deadenylation, and hence provide a mechanism for attenuating protein synthesis (PubMed:22701344, PubMed:22367205, PubMed:25505318, PubMed:24830504, PubMed:27102483). Acts as a 3'-untranslated region (UTR) ARE mRNA-binding adapter protein to communicate signaling events to the mRNA decay machinery (By similarity). Functions by recruiting the CCR4-NOT deadenylase complex and probably other components of the cytoplasmic RNA decay machinery to the bound ARE-containing mRNAs, and hence promotes ARE-mediated mRNA deadenylation and decay processes (By similarity). Binds to 3'-UTR ARE of numerous mRNAs (PubMed:22701344, PubMed:22367205, PubMed:25505318, PubMed:24830504). Promotes ARE-containing mRNA decay of the low-density lipoprotein (LDL) receptor (LDLR) mRNA in response to phorbol 12-myristate 13-acetate (PMA) treatment in a p38 MAPK-dependent manner (By similarity). Positively regulates early adipogenesis by promoting ARE-mediated mRNA decay of immediate early genes (IEGs) (PubMed:22701344). Plays a role in mature peripheral neuron integrity by promoting ARE-containing mRNA decay of the transcriptional repressor REST mRNA (PubMed:25505318). Plays a role in ovulation and oocyte meiotic maturation by promoting ARE-mediated mRNA decay of the luteinizing hormone receptor LHCGR mRNA (PubMed:24830504). Acts as a negative regulator of erythroid cell differentiation: promotes glucocorticoid-induced self-renewal of erythroid cells by binding mRNAs that are induced or highly expressed during terminal erythroid differentiation and promotes their degradation, preventing erythroid cell differentiation (PubMed:19633199, PubMed:23748442). In association with ZFP36L1 maintains quiescence on developing B lymphocytes by promoting ARE-mediated decay of several mRNAs encoding cell cycle regulators that help B cells progress through the cell cycle, and hence ensuring accurate variable-diversity-joining (VDJ) recombination process and functional immune cell formation (PubMed:27102483). Together with ZFP36L1 is also necessary for thymocyte development and prevention of T-cell acute lymphoblastic leukemia (T-ALL) transformation by promoting ARE-mediated mRNA decay of the oncogenic transcription factor NOTCH1 mRNA (PubMed:20622884). {ECO:0000250|UniProtKB:P47974, ECO:0000269|PubMed:19633199, ECO:0000269|PubMed:20622884, ECO:0000269|PubMed:22367205, ECO:0000269|PubMed:22701344, ECO:0000269|PubMed:23748442, ECO:0000269|PubMed:24830504, ECO:0000269|PubMed:25505318, ECO:0000269|PubMed:27102483}.

Molecular Weight: 50.1 kDa

UniProt: P23949

Pathways: Stem Cell Maintenance