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Ret Proto-Oncogene (RET) (AA 709-1018) protein (rho-1D4 tag)

Details for Product No. ABIN3133865, Supplier: Log in to see
Protein Name
  • c-ret
  • cret
  • etID315074.13
  • ret1
  • wu:fd13h01
  • X-ret
  • ret-A
  • xret
  • RET
  • MTC1
  • CDHF12
  • CDHR16
  • HSCR1
  • MEN2A
  • MEN2B
  • PTC
  • RET-ELE1
  • RET51
  • RET9
  • c-Ret
  • ret proto-oncogene receptor tyrosine kinase
  • ret proto-oncogene
  • ret
  • RET
  • Ret
Protein Characteristics
AA 709-1018
15
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Origin
Mouse (Murine)
28
4
Source
Insect Cells
15
11
2
2
1
1
Protein Type
Recombinant
Purification tag / Conjugate
This Ret Proto-Oncogene protein is labelled with rho-1D4 tag.
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Options
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Sequence SFKIPEDPKW EFPRKNLVLG KTLGEGEFGK VVKATAFRLK GRAGYTTVAV KMLKENASQS ELRDLLSEFN LLKQVNHPHV IKLYGACSQD GPLLLIVEYA KYGSLRGFLR DSRKIGPAYV SGGGSRNSSS LDHPDERVLT MGDLISFAWQ ISRGMQYLAE MKLVHRDLAA RNILVAEGRK MKISDFGLSR DVYEEDSYVK KSKGRIPVKW MAIESLFDHI YTTQSDVWSF GVLLWEIVTL GGNPYPGIPP ERLFNLLKTG HRMERPDNCS EEMYRLMLQC WKQEPDKRPV FADISKDLEK MMVKSRDYLD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Characteristics
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Ret Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
Purification Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
  1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
  2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
  3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Purity >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterility 0.22 μm filtered
Endotoxin Level Protein is endotoxin-free.
Grade Crystallography grade
Background Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. Phosphorylates PTK2/FAK1. Regulates both cell death/survival balance and positional information. Required for the molecular mechanisms orchestration during intestine organogenesis, involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner. Involved in the development of the neural crest. Active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage. Acts as a dependence receptor, in the presence of the ligand GDNF in somatotrophs (within pituitary), promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. Regulates nociceptor survival and size. Triggers the differentiation of rapidly adapting (RA) mechanoreceptors. Mediator of several diseases such as neuroendocrine cancers, these diseases are characterized by aberrant integrins-regulated cell migration. {ECO:0000269|PubMed:17322904, ECO:0000269|PubMed:20237269}.
Molecular Weight 36.6 kDa Including tag.
UniProt P35546
Research Area Kinases/Phosphatases, Signaling, Tyrosine Kinases, Cancer
Pathways RTK Signaling, Dopaminergic Neurogenesis, Regulation of Cell Size, Tube Formation
Application Notes In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Comment

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions For Research Use only
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -80 °C
Storage Comment Store at -80°C.
Expiry Date Unlimited (if stored properly)
Supplier Images
 image for Ret Proto-Oncogene (RET) (AA 709-1018) protein (rho-1D4 tag) (ABIN3133865) „Crystallography Grade“ protein due to multi-step, protein-specific purification process