FOXA3 Protein (AA 1-353) (Strep Tag)

Catalog No. ABIN3133875

Product Details

Target: FOXA3 (Forkhead Box A3 (FOXA3))

Protein Type: Recombinant

Protein Characteristics: AA 1-353

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This FOXA3 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MLGSVKMEAH DLAEWSYYPE AGEVYSPVNP VPTMAPLNSY MTLNPLSSPY PPGGLQASPL PTGPLAPPAP TAPLGPTFPS LGTGGSTGGS ASGYVAPGPG LVHGKEMAKG YRRPLAHAKP PYSYISLITM AIQQAPGKML TLSEIYQWIM DLFPYYRENQ QRWQNSIRHS LSFNDCFVKV ARSPDKPGKG SYWALHPSSG NMFENGCYLR RQKRFKLEEK AKKGNSATSA SRNGTAGSAT SATTTAATAV TSPAQPQPTP SEPEAQSGDD VGGLDCASPP SSTPYFSGLE LPGELKLDAP YNFNHPFSIN NLMSEQTSTP SKLDVGFGGY GAESGEPGVY YQSLYSRSLL NAS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: FOXA3 (Forkhead Box A3 (FOXA3))

Alternative Name: Foxa3 (FOXA3 Products)

Synonyms: FOXA3 Protein, Zffkh1 Protein, fa11c04 Protein, fkd2 Protein, forkhead-2 Protein, wu:fa11c04 Protein, wu:fc37a08 Protein, zf-FKH1 Protein, FKHH3 Protein, HNF3G Protein, TCF3G Protein, HNF3-G Protein, Hnf-3g Protein, Hnf3g Protein, Tcf-3g Protein, Tcf3g Protein, forkhead box A3 Protein, FOXA3 Protein, foxa3 Protein, Foxa3 Protein

Background: Hepatocyte nuclear factor 3-gamma (HNF-3-gamma) (HNF-3G) (Forkhead box protein A3),FUNCTION: Transcription factor that is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites (By similarity). Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis, activates GLUT2 transcription. Involved in regulation of neuronal-specific transcription. Involved in regulation of spermatogenesis, required for the maintenance of the testicular germ cell population and male fertility. {ECO:0000250, ECO:0000269|PubMed:11546810, ECO:0000269|PubMed:17488644, ECO:0000269|PubMed:9632808}.

Molecular Weight: 37.6 kDa

UniProt: P35584

Pathways: Carbohydrate Homeostasis