SULT1E1 Protein (AA 1-295) (Strep Tag)

Catalog No. ABIN3134279

Product Details

Target: SULT1E1 (Sulfotransferase Family 1E Member 1 (SULT1E1))

Protein Type: Recombinant

Protein Characteristics: AA 1-295

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This SULT1E1 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: METSMPEYYE VFGEFRGVLM DKRFTKYWED VEMFLARPDD LVIATYPKSG TTWISEVVYM IYKEGDVEKC KEDAIFNRIP YLECRNEDLI NGIKQLKEKE SPRIVKTHLP PKLLPASFWE KNCKMIYLCR NAKDVAVSYY YFLLMITSYP NPKSFSEFVE KFMQGQVPYG SWYDHVKAWW EKSKNSRVLF MFYEDMKEDI RREVVKLIEF LERKPSAELV DRIIQHTSFQ EMKNNPSTNY TMMPEEMMNQ KVSPFMRKGI IGDWKNHFPE ALRERFDEHY KQQMKDCTVK FRMEL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SULT1E1 (Sulfotransferase Family 1E Member 1 (SULT1E1))

Alternative Name: Sult1e1 (SULT1E1 Products)

Synonyms: STE Protein, St1 Protein, EST Protein, ST1E1 Protein, Ste Protein, EST-1 Protein, est-1 Protein, ste Protein, sult1e1 Protein, sult1e1.S Protein, sulfotransferase family 1E member 1 Protein, sulfotransferase family 1E, estrogen-preferring, member 1 Protein, estrogen sulfotransferase Protein, sulfotransferase family 1E, member 1 Protein, sulfotransferase family 1E member 1 L homeolog Protein, Sult1e1 Protein, SULT1E1 Protein, St1 Protein, CpipJ_CPIJ012993 Protein, CpipJ_CPIJ014515 Protein, LOC482182 Protein, sult1e1.L Protein

Background: Sulfotransferase 1E1 (ST1E1) (EC 2.8.2.4) (Estrogen sulfotransferase, testis isoform) (Sulfotransferase, estrogen-preferring),FUNCTION: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone (By similarity) (PubMed:9765259). Is a key enzyme in estrogen homeostasis, the sulfation of estrogens leads to their inactivation. Also sulfates dehydroepiandrosterone, pregnenolone, (24S)-hydroxycholesterol and xenobiotic compounds like ethinylestradiol, equalenin, diethyl stilbesterol and 1-naphthol at significantly lower efficiency. Does not sulfonate cortisol, testosterone and dopamine (By similarity). May play a role in gut microbiota-host metabolic interaction. O-sulfonates 4-ethylphenol (4-EP), a dietary tyrosine-derived metabolite produced by gut bacteria. The product 4-EPS crosses the blood-brain barrier and may negatively regulate oligodendrocyte maturation and myelination, affecting the functional connectivity of different brain regions associated with the limbic system. {ECO:0000250|UniProtKB:P49888, ECO:0000269|PubMed:9765259}.

Molecular Weight: 35.6 kDa

UniProt: P49891

Pathways: Steroid Hormone Biosynthesis