TRIM71 Protein (AA 2-855) (His tag)

Catalog No. ABIN3134930

Product Details

Target: TRIM71 (Tripartite Motif Containing 71, E3 Ubiquitin Protein Ligase (TRIM71))

Protein Type: Recombinant

Protein Characteristics: AA 2-855

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This TRIM71 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: ASFPETDFQI CLLCKEMCGS PAPLSSNSSA SSSSSQTSTS SAGGGGPGAA ARRLHVLPCL HAFCRPCLEA HRLPAPGGAG PAEALKLRCP VCDQKVVLAE AAGMDALPSS AFLLSNLLDA VVATAEEPPP KNGRAGGGPG GAGGHSNHRH HAHHPAQRAA APAPQPPPGP AASPGSLLMR RPHGCSSCDE GNAASSRCLD CQEHLCDNCV RAHQRVRLTK DHYIERGPPG PAAASAAQQL GLGPPFAGAP FSILSVFPER LGFCQHHDDE VLHLYCDTCS VPICRECTLG RHGGHSFAYL QDALQDSRAL TIQLLADAQQ GRQALQLSIE QAQTVAEQVE MKAKVVQSEV KAVTARHKKA LEDRECELLW KVEKIRQVKA KSLFLQVEKL RQSLSKLEST ISAVQQVLEE GRALDILLAR DRMLAQVQEL KTIRGLLQPQ EDDRIMFTPP DQALYLALKS IGFVSSGAFA PLTKATGDGI KRALQGKVAS FTVMGYDHDG EPRHSGGDLM SVVVLGPDGN LFGAEVSDQQ NGTYIVSYRP QLEGEHLVSV TLYNQHIENS PFKVVVKSGR SYVGIGLPGL SFGSEGDGEG KLCRPWGVSV DKEGFIIVAD RSNNRIQVFK PCGSFHHKFG TLGSRPGQFD RPAGVACDAS RRIIVADKDN HRIQIFTFEG QFLLKFGEKG TKNGQFNYPW DVAVNSEGKI LVSDTRNHRI QLFGPDGVFL NKYGFEGSLW KHFDSPRGVA FNNEGHLVVT DFNNHRLLVI HPDCQSARFL GSEGSGNGQF LRPQGVAVDQ EGRIIVADSR NHRVQMFEAN GSFLCKFGAQ GSGFGQMDRP SGIAVTPDGL IVVVDFGNNR ILIF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Trim71 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: TRIM71 (Tripartite Motif Containing 71, E3 Ubiquitin Protein Ligase (TRIM71))

Alternative Name: Trim71 (TRIM71 Products)

Synonyms: LIN-41 Protein, LIN41 Protein, RGD1566388 Protein, 2610206G21Rik Protein, AL022943 Protein, Gm1127 Protein, Lin41 Protein, Ripply2 Protein, lin-41 Protein, mLin41 Protein, mlin-41 Protein, tripartite motif containing 71 Protein, tripartite motif-containing 71 Protein, TRIM71 Protein, Trim71 Protein

Background: E3 ubiquitin-protein ligase that cooperates with the microRNAs (miRNAs) machinery and promotes embryonic stem cells proliferation and maintenance. Binds to miRNAs and associates with AGO2, participating in post-transcriptional repression of transcripts such as CDKN1A. Facilitates the G1-S transition to promote rapid embryonic stem cell self-renewal by repressing CDKN1A expression. Required to maintain proliferation and prevent premature differentiation of neural progenitor cells during early neural development: positively regulates FGF signaling by controlling the stability of SHCBP1. {ECO:0000269|PubMed:19898466, ECO:0000269|PubMed:22508726, ECO:0000269|PubMed:22735451}.

Molecular Weight: 92.9 kDa Including tag.

UniProt: Q1PSW8

Pathways: Tube Formation