URI1 Protein (AA 1-531) (Strep Tag)

Catalog No. ABIN3134978

Product Details

Target: URI1 (URI1, Prefoldin-Like Chaperone (URI1))

Protein Type: Recombinant

Protein Characteristics: AA 1-531

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This URI1 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MEPPSEPEPE PQPLAEASAA APLRAPEVAR LREEQEKVVT NCQEKIQHWE KVDNDYSALQ ERLRTLPDKL SYDVMVPFGP LAFMPGKLVH TNEVTVLLGD NWFAKCSAKQ AVGLVEHRKE HVRKTIDDFK KVLKNFESRV EFTEDLQKMS DAAGDFVDIR EEIKSDFEFK GKQRIAHKPH SKPKTSDIFE ADFENGVKPK NTFDADELWA RLEELERQEE LLGELESKPD TVIANGEDRV SSEEEKEGAD TGVNVVSPVT DSSAASSCKR RAGNAGLPNG QVNSLNYSVN GSNSYHSNKD DDEEEEDDDD DDDEDDDNES DHAISADNSI PTIYFSHTVE PKRVRINTGK NTTLKFSEKK EEAKRKRKSG AGSHATHELP AIKSPADIYR VFVDVVNGEY VPRKSILKSR SRENSVCSDT SESSAADVED RRGLLRSTSS EEAVATEAGG SSLDELQENH PKKPLPSGVS EAFSGTVIEK EFLSPSLAPY SAIAHHALPT IPERKEVPSE VSEEPTKRVS KFRAARLQQR S
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: URI1 (URI1, Prefoldin-Like Chaperone (URI1))

Alternative Name: Uri1 (URI1 Products)

Synonyms: c19orf2 Protein, C19orf2 Protein, NNX3 Protein, PPP1R19 Protein, RMP Protein, URI Protein, URI1 prefoldin-like chaperone L homeolog Protein, URI1, prefoldin like chaperone Protein, uri1.L Protein, URI1 Protein

Background: Unconventional prefoldin RPB5 interactor (Protein NNX3) (Protein phosphatase 1 regulatory subunit 19) (RNA polymerase II subunit 5-mediating protein) (RPB5-mediating protein),FUNCTION: Involved in gene transcription regulation. Acts as a transcriptional repressor in concert with the corepressor UXT to regulate androgen receptor (AR) transcription. May act as a tumor suppressor to repress AR-mediated gene transcription and to inhibit anchorage-independent growth in prostate cancer cells. Required for cell survival in ovarian cancer cells. Together with UXT, associates with chromatin to the NKX3-1 promoter region (By similarity). {ECO:0000250}., FUNCTION: Plays a central role in maintaining S6K1 signaling and BAD phosphorylation under normal growth conditions thereby protecting cells from potential deleterious effects of sustained S6K1 signaling. The URI1-PPP1CC complex acts as a central component of a negative feedback mechanism that counteracts excessive S6K1 survival signaling to BAD in response to growth factors. Mediates inhibition of PPP1CC phosphatase activity in mitochondria. Coordinates the regulation of nutrient-sensitive gene expression availability in a mTOR-dependent manner. Seems to be a scaffolding protein able to assemble a prefoldin-like complex that contains PFDs and proteins with roles in transcription and ubiquitination (By similarity). {ECO:0000250}.

Molecular Weight: 59.1 kDa

UniProt: Q3TLD5