Peroxidasin Protein (AA 24-1475) (His tag)
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- Target See all Peroxidasin (PXDN) Proteins
- Peroxidasin (PXDN) (Peroxidasin Homolog (PXDN))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 24-1475
- Origin
- Mouse
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Source
- Insect Cells
- Purification tag / Conjugate
- This Peroxidasin protein is labelled with His tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- Sequence
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VVASKQGAGC PSRCLCFRTT VRCMHLLLEA VPAVAPQTSI LDLRFNRIRE IQPGAFRRLR SLNTLLLNNN QIKKIPNGAF EDLENLKYLY LYKNEIQSID RQAFKGLASL EQLYLHFNQI ETLDPESFQH LPKLERLFLH NNRITHLVPG TFSQLESMKR LRLDSNALHC DCEILWLADL LKTYAQSGNA QAAATCEYPR RIQGRSVATI TPEELNCERP RITSEPQDAD VTSGNTVYFT CRAEGNPKPE IIWLRNNNEL SMKTDSRLNL LDDGTLMIQN TQEADEGVYQ CMAKNVAGEA KTQEVTLRYL GSPARPTFVI QPQNTEVLVG ESVTLECSAT GHPLPQITWT RGDRTPLPID PRVNITPSGG LYIQNVAQSD SGEYTCFASN SVDSIHATAF IIVQALPQFT VTPQSRVVIE GQTVDFQCAA KGHPQPVIAW TKGGSQLSVD RRHLVLSSGT LRISGVALHD QGQYECQAVN IIGSQKVVAH LTVQPRVTPV FASIPSDMTV EVGTNVQLPC SSQGEPEPAI TWNKDGVQVT ESGKFHISPE GFLTINDVGT ADAGRYECVA RNTIGYASVS MVLSVNVPDV SRNGDPYVAT SIVEAIATVD RAINSTRTHL FDSRPRSPND LLALFRYPRD PYTVGQARAG EIFERTLQLI QEHVQHGLMV DLNGTSYHYN DLVSPQYLSL IANLSGCTAH RRVNNCSDMC FHQKYRTHDG TCNNLQHPMW GASLTAFERL LKAVYENGFN TPRGINSQRQ YNGHVLPMPR LVSTTLIGTE VITPDEQFTH MLMQWGQFLD HDLDSTVVAL SQARFSDGQH CSSVCSNDPP CFSVMIPPND PRVRSGARCM FFVRSSPVCG SGMTSLLMNS VYPREQINQL TSYIDASNVY GSTDHEARSI RDLASHRGLL RQGIVQRSGK PLLPFATGPP TECMRDENES PIPCFLAGDH RANEQLGLTS MHTLWFREHN RIAAELLKLN PHWDGDTVYH ETRKIVGAEI QHITYRHWLP KILGEVGMKM LGEYRGYDPS VNAGIFNAFA TAAFRFGHTL INPLLYRLDE NFEPIPQGHV PLHKAFFSPF RIVNEGGIDP LLRGLFGVAG KMRIPSQLLN TELTERLFSM AHTVALDLAA INIQRGRDHG IPPYHDYRVY CNLSAAYTFE DLKNEIKSPV IREKLQRLYG STLNIDLFPA LMVEDLVPGS RLGPTLMCLL STQFRRLRDG DRLWYENPGV FSPAQLTQLK QTSLARILCD NSDNITRVQQ DVFRVAEFPH GYSSCEDIPR VDLRVWQDCC EDCRTRGQFN AFSYHFRGRR SLEFSYEDDK PTKRARWRKA LSVKHGKHLS NATSATHEHL EGPATNDLKE FVLEMQKIIT DLRKQINSLE SRLSTTECVD DSGESHGGNT KWKKDPCTVC ECKNGQITCF VEACQPAACP QPVKVEGACC PVCLKNTAEE KP
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Pxdn Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product PXDN Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- Peroxidasin (PXDN) (Peroxidasin Homolog (PXDN))
- Alternative Name
- Pxdn (PXDN Products)
- Synonyms
- D2S448 Protein, D2S448E Protein, MG50 Protein, PRG2 Protein, PXN Protein, VPO Protein, 2310075M15Rik Protein, C85409 Protein, E330004E07 Protein, VPO1 Protein, mKIAA0230 Protein, peroxidasin Protein, PXDN Protein, Pxdn Protein
- Background
- Displays low peroxidase activity and is likely to participate in H(2)O(2) metabolism and peroxidative reactions in the cardiovascular system (By similarity). Plays a role in extracellular matrix formation. {ECO:0000250}.
- Molecular Weight
- 163.4 kDa Including tag.
- UniProt
- Q3UQ28
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