RBM20 Protein (AA 1-1199) (Strep Tag)

Catalog No. ABIN3135080

Product Details

Target: RBM20 (RNA Binding Motif Protein 20 (RBM20))

Protein Type: Recombinant

Protein Characteristics: AA 1-1199

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This RBM20 protein is labelled with Strep Tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Sequence: MVLAVAMSQD ADPSGPEQPD RDACVMPGVQ GPSVPQGQQG MQPLPPPPPP QPQASLPQII QNAAKLLDKS PFSVNNQNPL LTSPASVQLA QIQAQLTLHR LKMAQTAVTN NTAAATVLNQ VLSKVAMSQP LFNQLRHPSV LGTAHGPTGV SQHAASVPSA HFPSTAIAFS PPSQTGGPGP SVSLPSQPPN AMVVHTFSGV VPQTPAQPAV ILSLGKAGPT PATTGFYDYG KANSGQAYGS ETEGQPGFLP ASASATASGS MTYEGHYSHT GQDGQPAFSK DFYGPNAQGP HIAGGFPADQ TGSMKGDVGG LLQGTNSQWE RPPGFSGQNK PDITAGPSLW APPASQPYEL YDPEEPTSDR APPAFGSRLN NSKQGFGCSC RRTKEGQAVL SVRPLQGHQL NDFRGLAPLH LPHICSICDK KVFDLKDWEL HVKGKLHAQK CLLFSESAGL RSIRASGEGT LSASANSTAV YNPTGNEDYT SNLGTSYAAI PTRAFAQSNP VFPSASSGTS FAAQRKGAGR VVHICNLPEG SCTENDVINL GLPFGKVTNY ILMKSTNQAF LEMAYTEAAQ AMVQYYQEKP AIINGEKLLI RMSTRYKELQ LKKPGKNVAA IIQDIHSQRE RDMLREADRY GPERPRSRSP MSRSLSPRSH SPPGPSRADW GNGRDSYAWR DEDRETVPRR ENGEDKRDRL DVWAHDRKHY PRQLDKAELD ERLEGGRGYR EKYLKSGSPG PLHSVSGYKG REDGYHRKEP KAKLDKYPKQ QPDVPGRSRR KEEARLREPR HPHPEDSGKA EDLEPKITRA PDGTKSKQSE KSKTKRADRD QEGADDKKES QLAENEAGAE EQEGMVGIQQ EGTESCDPEN TRTKKGQDCD SGSEPEGDNW YPTNMEELVT VDEVGEEDFI MEPDLPELEE IVPIDQKDKT LPKICTCVTA TLGLDLAKDF TKQGETLGNG DAELSLKLPG QVPSTSASCP NDTDLEMPGL NLDAERKPAE SETGLSLEVS NCYEKEARGE EDSDVSLAPA VQQMSSPQPA DERARQSSPF LDDCKARGSP EDGSHEASPL EGKASPPTES DLQSQACREN PRYMEVKSLN VRSPEFTEAE LKEPLSLPSW EPEVFSELSI PLGVEFVVPR TGFYCKLCGL FYTSEEAAKV SHCRSTVHYR NLQKYLSQLA EEGLKETEGT DSPSPERGGI GPHLERKKL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: RBM20 (RNA Binding Motif Protein 20 (RBM20))

Alternative Name: Rbm20 (RBM20 Products)

Synonyms: im:7146943 Protein, 1110018J23Rik Protein, 2010003H22Rik Protein, AI646761 Protein, RNA binding motif protein 20 Protein, RBM20 Protein, rbm20 Protein, Rbm20 Protein

Background: RNA-binding protein 20 (RNA-binding motif protein 20),FUNCTION: RNA-binding protein that acts as a regulator of mRNA splicing of a subset of genes encoding key structural proteins involved in cardiac development, such as TTN (Titin), CACNA1C, CAMK2D or PDLIM5/ENH (PubMed:22466703, PubMed:24584570, PubMed:27630136, PubMed:29650543, PubMed:29895960, PubMed:33110103, PubMed:35394688, PubMed:35041844). Acts as a repressor of mRNA splicing: specifically binds the 5'UCUU-3' motif that is predominantly found within intronic sequences of pre-mRNAs, leading to the exclusion of specific exons in target transcripts (PubMed:32187365). RBM20-mediated exon skipping is hormone-dependent and is essential for TTN isoform transition in both cardiac and skeletal muscles (PubMed:27630136, PubMed:29895960, PubMed:33110103, PubMed:35041844). RBM20-mediated exon skipping of TTN provides substrates for the formation of circular RNA (circRNAs) from the TTN transcripts (PubMed:37272356). Together with RBM24, promotes the expression of short isoforms of PDLIM5/ENH in cardiomyocytes (By similarity). {ECO:0000250|UniProtKB:E9PT37, ECO:0000250|UniProtKB:Q5T481, ECO:0000269|PubMed:22466703, ECO:0000269|PubMed:24584570, ECO:0000269|PubMed:27630136, ECO:0000269|PubMed:29650543, ECO:0000269|PubMed:29895960, ECO:0000269|PubMed:32187365, ECO:0000269|PubMed:33110103, ECO:0000269|PubMed:35041844, ECO:0000269|PubMed:35394688, ECO:0000269|PubMed:37272356}.

Molecular Weight: 130.1 kDa

UniProt: Q3UQS8