Nesprin3 Protein (AA 1-925) (His tag)

Catalog No. ABIN3135137

Product Details

Target: Nesprin3 (C14orf49) (Chromosome 14 Open Reading Frame 49 (C14orf49))

Protein Type: Recombinant

Protein Characteristics: AA 1-925

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This Nesprin3 protein is labelled with His tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)

Sequence: MTQQPQEDFE RSVEDAQAWM KVIQEQLQVN DNTKGPRAAL EARLRETEKI CQLESEGMVK VELVLRAAEA LLATCQEGQK PEILARLRDI KSQWEETVTY MTHCHSRIEW VWLHWSEYLL AQDEFYRWFQ KMVVALEPPV ELQLGLKEKQ WQLSHAQVLL HNVDNQAVLL DRLLEEAGSL FSRIGDPSVD EDAQKRMKAE YDAVKARAQR RVDLLAQVAQ DHEQYREDVN EFQLWLKAVV EKVHSCLGRN CKLATELRLS TLQDIAKDFP RGEESLKRLE EQAVGVIQNT SPLGAEKISG ELEEMRGVLE KLRVLWKEEE GRLRGLLQSR GDCEQQIQQL EAELGDFKKS LQRLAQEGLE PTVKTATEDE LVAQWRLFSG TRAALASEEP RVDRLQTQLK KLVTFPDLQS LSDSVVATIQ EYQSMKGKNT RLHNATRAEL WQRFQRPLND LQLWKALAQR LLDITASLPD LASIHTFLPQ IEAALTESSR LKEQLAMLQL KTDLLGSIFG QERAATLLEQ VTSSVRDRDL LHNSLLQRKS KLQSLLVQHK DFGVAFDPLN RKLLDLQARI QAEKGLPRDL PGKQVQLLRL QGLQEEGLDL GTQIEAVRPL AHGNSKHQQK VDQISCDQQA LQRSLEDLVD RCQQNVREHC TFSHRLSELQ LWITMATQTL ESHQGDVRLW DAESQEAGLE TLLSEIPEKE VQVSLLQALG QLVMKKSSPE GATMVQEELR KLMESWQALR LLEENMLSLM RNQQLQRTEV DTGKKQVFTN NIPKAGFLIN PQDPIPRRQH GANPLEGHDL PEDHPQLLRD FEQWLQAENS KLRRIITMRV ATAKDLRTRE VKLQELEARI PEGQHLFENL LRLRPARDPS NELEDLRYRW MLYKSKLKDS GHLLTESSPG ELTAFQKSRR QKRWSPCSLL QKACR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Syne3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Nesprin3 (C14orf49) (Chromosome 14 Open Reading Frame 49 (C14orf49))

Alternative Name: Syne3 (C14orf49 Products)

Synonyms: C14orf49 Protein, NET53 Protein, Nesp3 Protein, 4831426I19Rik Protein, nesprin-3 Protein, spectrin repeat containing nuclear envelope family member 3 Protein, spectrin repeat containing, nuclear envelope family member 3 Protein, SYNE3 Protein, Syne3 Protein

Background: Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow-induced centrosome polarization and directional migration in aortic endothelial cells (By similarity). {ECO:0000250, ECO:0000269|PubMed:16330710}.

Molecular Weight: 107.5 kDa Including tag.

UniProt: Q4FZC9

Pathways: Maintenance of Protein Location