PHACTR4 Protein (AA 1-694) (Strep Tag)

Catalog No. ABIN3135154

Product Details

Target: PHACTR4 (Phosphatase and Actin Regulator 4 (PHACTR4))

Protein Type: Recombinant

Protein Characteristics: AA 1-694

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This PHACTR4 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MEDPSEEAEQ PSGDPGMGMD SVEAGDTTPP TKRKSKFSAL GKIFKPWKWR KKKSSDKFKE TSEVLERKIS MRKPREELVK RGVLLEDPEQ DGEDSGKLSH AALKNGHTTP IGSARSSSPV LVEEEPERSL RNLTPEEESK KRLGSTGSQP NSEAEPGPEH APKQPLLPPK RPLSSSCEAK EVPAGSTARS VSSTSGSTTV TSAATTAATD MTKTVKSFVG PTPAPAPAPR TLPAAPASAN TAATTTAPAK QPPIPPPKPA QRNSNPIIAE LSQAMNSGTV LSKPSPPLPP KRGIPSTSIP SLEPAASFTT KTANDQREKT VSLCLEPPLI IPPSSPSPPL PTHIPPEPPR SPLVPAKTFQ IVPEVEFSSS SDLFQDISQQ EDQKTEVPKK IQDQSFGESH IPSRLPPLPL HIRIQQALTS PLPVTPPLEG THRAHSLLFE NSDSFSEDTG TLGRTRSLPI TIEMLKVPDD EEEEQTCPFV EDVTSTSATP SLPLCLREEE KESDSDSEGP IKYRDEEEDD DDDESHQSAL ANRVKRKDTL AMKLSSRPSE PETNLNSWPR KSKEEWNEIR HQIGNTLIRR LSQRPTAEEL EQRNILQPKN EADRQAEKRE IKRRLTRKLS QRPTVAELLA RKILRFNEYV EVTDAHDYDR RADKPWTKLT PADKAAIRKE LNEFKSSEME VHVDSKHFTR YHRP
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PHACTR4 (Phosphatase and Actin Regulator 4 (PHACTR4))

Alternative Name: Phactr4 (PHACTR4 Products)

Synonyms: 3110001B12Rik Protein, AI527228 Protein, AW495572 Protein, C330013F19Rik Protein, N28169 Protein, mKIAA4120 Protein, phactr4 Protein, zgc:63484 Protein, zgc:123224 Protein, phosphatase and actin regulator 4 Protein, phosphatase and actin regulator 4b Protein, phosphatase and actin regulator 4 L homeolog Protein, phosphatase and actin regulator 4 S homeolog Protein, phosphatase and actin regulator 4a Protein, Phactr4 Protein, phactr4b Protein, PHACTR4 Protein, phactr4.L Protein, phactr4.S Protein, Tsp_08786 Protein, phactr4 Protein, phactr4a Protein

Background: Phosphatase and actin regulator 4 (Protein Humpty dumpty) (Humdy),FUNCTION: Regulator of protein phosphatase 1 (PP1) required for neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. Acts as an activator of PP1 by interacting with PPP1CA and preventing phosphorylation of PPP1CA at 'Thr-320'. During neural tube closure, localizes to the ventral neural tube and activates PP1, leading to down-regulate cell proliferation within cranial neural tissue and the neural retina. Also acts as a regulator of migration of enteric neural crest cells (ENCCs) by activating PP1, leading to dephosphorylation and subsequent activation of cofilin (COF1 or COF2) and repression of the integrin signaling through the RHO/ROCK pathway. {ECO:0000269|PubMed:17609112, ECO:0000269|PubMed:22215812}.

Molecular Weight: 76.6 kDa

UniProt: Q501J7

Pathways: Tube Formation