RNF20 Protein (AA 1-973) (Strep Tag)

Catalog No. ABIN3135178

Product Details

Target: RNF20 (Ring Finger Protein 20 (RNF20))

Protein Type: Recombinant

Protein Characteristics: AA 1-973

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This RNF20 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MSGIGNKRAA GEPGTSMPPE KKTAVEDSGT TVETIKLGGV SSTEELDIRT LQSKNRKLAE MLDQRQAIED ELREHIEKLE RRQATDDASL LIVNRYWSQF DENIRIILKR YDLDQGLGDL LTERKALVVP EPEPDSDSNQ ERKDDRERGD GQEPAFSFLA TLASSSSEEM ESQLQERVES SRRAVSQIVT VYDKLQEKVD LLSRKLNSGD NLIVEEAVQE LNSFLAQENV RLQELTDLLQ EKHHTMSQEF CKLQGKVETA ESRVSVLESM IDDLQWDIDK IRKREQRLNR HLAEVLERVN SKGYKVYGAG SSLYGGTITI NARKFEEMNA ELEENKELAQ NRHCELEKLR QDFEEVTTQN EKLKVELRSA VEEVVKETPE YRCMQSQFSV LYNESLQLKA HLDEARTLLH GTRGTHQRQV ELIERDEVSL HKKLRTEVIQ LEDTLAQVRK EYEMLRIEFE QTLAANEQAG PINREMRHLI SSLQNHNHQL KGEVLRYKRK LREAQSDLNK TRLRSGSALL QSQSSTEDPK DEPTELKQDS EDLATHSSAL KASQEDEVKS KRDEEERERE RREKERERER EREKEKERER EKQKLKESEK ERDSVKDKEK GKHDDGRKKE AEIIKQLKIE LKKAQESQKE MKLLLDMYRS APKEQRDKVQ LMAAEKKSKA ELEDLRQRLK DLEDKEKKEN KKMADEDALR KIRAVEEQIE YLQKKLAMAK QEEEALLSEM DVTGQAFEDM QEQNIRLMQQ LREKDDANFK LMSERIKSNQ IHKLLKEEKE ELADQVLTLK TQVDAQLQVV RKLEEKEHLL QSNIGTGEKE LGLRTQALEM NKRKAMEAAQ LADDLKAQLE LAQKKLHDFQ DEIVENSVTK EKDLFNFKRA QEDISRLRRK LETTKKPDNV PKCDEILMEE IKDYKARLTC PCCNMRKKDA VLTKCFHVFC FECVKTRYDT RQRKCPKCNA AFGANDFHRI YIG
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: RNF20 (Ring Finger Protein 20 (RNF20))

Alternative Name: Rnf20 (RNF20 Products)

Synonyms: 4833430L21Rik Protein, AW540162 Protein, C79397 Protein, mKIAA4116 Protein, BRE1 Protein, BRE1A Protein, hBRE1 Protein, ring finger protein 20 Protein, RNF20 Protein, Rnf20 Protein

Background: E3 ubiquitin-protein ligase BRE1A (BRE1-A) (EC 2.3.2.27) (RING finger protein 20) (RING-type E3 ubiquitin transferase BRE1A),FUNCTION: Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1) (PubMed:28453857). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B, reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of PA2G4 leading to its proteasome-mediated degradation. {ECO:0000250|UniProtKB:Q5VTR2, ECO:0000269|PubMed:28453857}.

Molecular Weight: 113.5 kDa

UniProt: Q5DTM8