MTUS1 Protein (AA 1-1210) (Strep Tag)

Catalog No. ABIN3135196

Product Details

Target: MTUS1 (Microtubule Associated Tumor Suppressor 1 (MTUS1))

Protein Type: Recombinant

Protein Characteristics: AA 1-1210

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This MTUS1 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MNDDNSDRTE DGSRYVFIRD KNSNPSEYYQ TSLSAQCPSV SHGDWNSDNP DAMVVDYEMD PAVDSSESVS LSHQCVEELA YPEPSSDFMG KHEFTMYSEL TCQSPALVNT GKPQDLHSNC DSLEAIQDEK FDPLKPCECR SDDDYACGDS PEVLELKQTY GMKVDTANYT FIARHDIEQG QPLHAPGGLQ TTVRDRNALS SCGRTPPHSS KMYVRGVNYN RENFENLQAT PSKTLNTTFT VISDVLMQTD SPDVGVQGQN SLGNVTKEYT DGTRRGLIGE KEIQAVTLVS DGMEVPNGSA SQEFYCVSED DPNSETHSHG PYAQQEMGQN LRGTLPNCHV DGECPVLVPA FEKSKTRVLG SECKVTVTED PHIDSHDNDS DIQSSTEELT LRSVSGQRGS PYEMGWGENG GAICTDKAGC MSTPVEQPPN LSFRLEPAEV KKYNNVENGP RDAKRAPNLK GEPTNMPKPN LGKSATKTNT TVGSKVRKTE IISYPTPNFK NIKAKVISRS VLQPKDTSIM KDTPSPQVTG GSSPSPGPSK HLTMMNKAPR SDFKASKKAE IPINKTHKQQ FNKLITSQAA QVTTHSKNAS LGVPRTTSAT KSNQENVDKT GSPHAGSETG SVAAFFQKIK GILPVKMKSS ECLEVTYVSH IDQISPEKGE QDGEAPMEKQ ELGKQATNEI FESKSLLVGS APKTSTTPGR SSSKPDSRSL RKTPGLKAKV GPTAACLRRK SESRTLGSDR ALSPQRIRRV SGSGGHAAIN KYEEKPPKQA FQNGSGPLYL KPLVPRAHSH LLKTSPKGPS RKSLFTAFNS VEKGRQKNPR SLCIQTQTAP DVLSSERTLE LAQYKTKCES QSGFILHLRQ LLSRGNTKFE ALTVVIQHLL SEREEALKQH KTLSQELVSL RGELVAASSA CEKLEKARAD LQTAYQEFVQ KLNQQHQTDR TELENRLKDL YTAECEKLQS IYIEEAEKYK TQLQEQFDNL NAAHETTKLE IEASHSEKVE LLKKTYETSL SEIKKSHEME KKSLEDLLNE KQESLEKQIN DLKSENDALN ERLKSEEQKQ LSREKANSKN PQVMYLEQEL ESLKAVLEIK NEKLHQQDMK LMKMEKLVDN NTALVDKLKR FQQENEELKA RMDKHMAISR QLSTEQAALQ ESLEKESKVN KRLSMENEEL LWKLHNGDLC SPKRSPTSSA IPFQSPRNSG SFSSPSISPR
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: MTUS1 (Microtubule Associated Tumor Suppressor 1 (MTUS1))

Alternative Name: Mtus1 (MTUS1 Products)

Synonyms: ATBP Protein, ATIP Protein, ICIS Protein, MP44 Protein, MTSG1 Protein, AI481402 Protein, ATBP135 Protein, Atip1 Protein, B430010I23Rik Protein, B430305I03Rik Protein, C85752 Protein, Cctsg1-440 Protein, MD44 Protein, mKIAA1288 Protein, ATIP4 Protein, Atip3b Protein, Mtsg1 Protein, fbx27 Protein, fa17d11 Protein, wu:fa17d11 Protein, wu:fi18f08 Protein, zgc:154168 Protein, microtubule associated scaffold protein 1 Protein, mitochondrial tumor suppressor 1 Protein, microtubule associated scaffold protein 1 L homeolog Protein, microtubule associated tumor suppressor 1 Protein, microtubule associated tumor suppressor 1a Protein, MTUS1 Protein, Mtus1 Protein, mtus1.L Protein, mtus1a Protein

Background: Microtubule-associated tumor suppressor 1 homolog (AT2 receptor-binding protein) (Angiotensin-II type 2 receptor-interacting protein) (Coiled-coiled tumor suppressor gene 1 protein) (Mitochondrial tumor suppressor 1 homolog),FUNCTION: Cooperates with AGTR2 to inhibit ERK2 activation and cell proliferation. May be required for AGTR2 cell surface expression. Together with PTPN6, induces UBE2V2 expression upon angiotensin-II stimulation. {ECO:0000269|PubMed:15123706, ECO:0000269|PubMed:15539617, ECO:0000269|PubMed:17068200}.

Molecular Weight: 134.4 kDa

UniProt: Q5HZI1