Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

NSP5 Protein (AA 1-1067) (His tag)

Crystallography grade SPECC1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Catalog No. ABIN3135243
  • Target See all NSP5 (SPECC1) Proteins
    NSP5 (SPECC1) (Sperm Antigen with Calponin Homology and Coiled-Coil Domains 1 (SPECC1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-1067
    Origin
    • 3
    • 1
    Mouse
    Source
    • 2
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This NSP5 protein is labelled with His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequence
    MRSAAKPWSP VAKAGSHGPD RTRPLPGTPS GMKSSKSSTS LAFESRLSKL KRASSEDTLN KPGSASSGVA RLKKTSTSGA ISELTESRLR SNTGTIPTTK RTGIPAPREL SVTISRERSV PRGPSSSKKL GSSPTSSCNP TPTKHLRTTP AKPKQEHEGA EKAVLESQVR ELLAEAKTKD SEINRLRSEL KKCKERWALS TEDANASDPS AEGTASPESD AQPLIRTLEE KNKTFQKELA DLEEENRALK EKLTYLEQSP NSEGAASHTG DSSCPTSITH ESSFGSPVGN ELSSETDEYR RTTHGSALRT SGSSSSDVTK ASLSPDASDF EHITADTPSR PLSATSNPFK SSKGSPTGSS PNNASELSLA SLTEKIQKME ENQHSTAEEL QATLQELSDQ QQMVQELTAE NEKLVDEKTI LETSFHQHRE RAEQLSQENE KLINLLQERV KNEEPSAQGG KVLELEQKCT DILEKSRFER EKLLNIQQQL TCSLRKVEEE NQGAIDMIKH LKEENEKLNG FLEHERCNNS VMAKTLEECR VTLEGLKMEN GSLKALLEAD KQKAIEASST VGQTAENFEV QEMLKVARAE KDQLQLSCTE LRQELLKANG EIKHVSSLLA KMEKDYSYLK EVCDHQAEQL SRTSLKLQEK ASESDAEIKD MKETIFELED QVEQHRAVKL HNNQLISELE GSVIKLEEQK SDLERQLKTL TKQIKEETEE WRRFQADLQT AVVVANDIKC EAQQELRTVK RRLLEEEEKN ARLQKELGDI QGHSRWVTGR ATLLPVNEEP EPSEADAAGR WRGVYVNRTS PAPSDSATTV KSLIKSFDLG HSGGTGQSIS VHKTPRSPLS GIPVRTAPAA AVSPMQRHST YSSMKPASKG TSQRLDLPDL PLSDLLKGRA EDRKSDPYLR KSPSLESLSR PPSLGFGNTR LLSASTGGLK PSKLSVERRD PLAALAREYG GSKRNALLKW CQKKTEGYAN IDITNFSSSW SDGLALCALL HTYLPAHIPY QELNSQEKKR NLLLAFEAAQ SVGINPSLEL SEMLYTDRPD WQSVMQYVAQ IYKYFET
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Specc1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product SPECC1 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    NSP5 (SPECC1) (Sperm Antigen with Calponin Homology and Coiled-Coil Domains 1 (SPECC1))
    Alternative Name
    Specc1 (SPECC1 Products)
    Synonyms
    CYTSB Protein, HCMOGT-1 Protein, HCMOGT1 Protein, NSP Protein, Cytsb Protein, RGD1309718 Protein, specc1 Protein, 2810012G08Rik Protein, B230396K10Rik Protein, SPECC1 Protein, si:dkey-13b16.2 Protein, sperm antigen with calponin homology and coiled-coil domains 1 Protein, sperm antigen with calponin homology and coiled-coil domains 1 L homeolog Protein, cytospin-B Protein, SPECC1 Protein, Specc1 Protein, specc1.L Protein, specc1 Protein, LOC100013871 Protein
    Molecular Weight
    119.1 kDa Including tag.
    UniProt
    Q5SXY1
You are here:
Support