HCFC1 Protein (AA 1433-2045) (His tag)

Catalog No. ABIN3135377

Product Details

Target: HCFC1 (Host Cell Factor C1 (VP16-Accessory Protein) (HCFC1))

Protein Type: Recombinant

Protein Characteristics: AA 1433-2045

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This HCFC1 protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: THETGTTHTA TTVTSNMSSN QDPPPAASDQ GEVASTQGDS TNITSASAIT TSVSSTLPRA VTTVTQSTPV PGPSVPPPEE LQVSPGPRQQ LPPRQLLQSA STPLMGESTE VLSASQTPEL QAAVDLSSTG DPSSGQEPTT SAVVATVVVQ PPPPTQSEVD QLSLPQELMA EAQAGTTTLM VTGLTPEELA VTAAAEAAAQ AAATEEAQAL AIQAVLQAAQ QAVMGTGEPM DTSEAAAAVT QAELGHLSAE GQEGQATTIP IVLTQQELAA LVQQQQQLQE AQAQAQQQHH LPTEALAPAD SLNDPSIESN CLNELASAVP STVALLPSTA TESLAPSNTF VAPQPVVASP AKMQAAATLT EVANGIESLG VKPDLPPPPS KAPVKKENQW FDVGVIKGTS VMVTHYFLPP DDAVQSDDDS GTVPDYNQLK KQELQPGTAY KFRVAGINAC GRGPFSEISA FKTCLPGFPG APCAIKISKS PDGAHLTWEP PSVTSGKIIE YSVYLAIQSS QASGEPKSST PAQLAFMRVY CGPSPSCLVQ SSSLSNAHID YTTKPAIIFR IAARNEKGYG PATQVRWLQE TSKDSSGTKP ASKRPMSSPE MKSAPKKSKA DGQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Hcfc1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: HCFC1 (Host Cell Factor C1 (VP16-Accessory Protein) (HCFC1))

Alternative Name: Hcfc1 (HCFC1 Products)

Synonyms: CFF Protein, HCF-1 Protein, HCF1 Protein, HFC1 Protein, MRX3 Protein, VCAF Protein, AW060991 Protein, HCF Protein, RGD1563804 Protein, fc61g09 Protein, hcfc1 Protein, im:7159458 Protein, wu:fc61g09 Protein, wu:fj40c12 Protein, HCFC1 Protein, host cell factor C1 Protein, host cell factor C1a Protein, host cell factor Protein, host cell factor C1 (VP16-accessory protein) Protein, HCFC1 Protein, Hcfc1 Protein, hcfc1a Protein, LOC5571204 Protein, CpipJ_CPIJ014908 Protein

Background: Involved in control of the cell cycle. Also antagonizes transactivation by ZBTB17 and GABP2, represses ZBTB17 activation of the p15(INK4b) promoter and inhibits its ability to recruit p300. Coactivator for EGR2 and GABP2. Tethers the chromatin modifying Set1/Ash2 histone H3 'Lys-4' methyltransferase (H3K4me) and Sin3 histone deacetylase (HDAC) complexes (involved in the activation and repression of transcription respectively) together. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. {ECO:0000269|PubMed:9334261, ECO:0000269|PubMed:9990006}.

Molecular Weight: 64.4 kDa Including tag.

UniProt: Q61191