SEMA5A Protein (AA 22-971) (His tag)

Catalog No. ABIN3135528

Product Details

Target: SEMA5A (Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A))

Protein Type: Recombinant

Protein Characteristics: AA 22-971

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This SEMA5A protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: PETQDPAKCQ RAEHPVVSYK EIGPWLREFR AENAVDFSRL TFDPGQKELV VGARNYLFRL ELEDLSLIQA VEWECDEATK KACYSKGKSK EECQNYIRVL LVGGDRLFTC GTNAFTPVCT IRSLSNLTEI HDQISGMARC PYSPQHNSTA LLTASGELYA ATAMDFPGRD PAIYRSLGTL PPLRTAQYNS KWLNEPNFVS SYDIGNFTYF FFRENAVEHD CGKTVFSRPA RVCKNDIGGR FLLEDTWTTF MKARLNCSRP GEVPFYYNEL QGTFFLPELD LIYGIFTTNV NSIASSAVCV FNLSAISQAF NGPFKYQENS RSAWLPYPNP NPNFQCGTMD QGLYVNLTER NLQDAQKFIL MHEVVQPVTT VPSFMEDNSR FSHLAVDVVQ GRETLVHIIY LGTDYGTIKK VRAPLSQSSG SCLLEEIELF PERRSEPIRS LQILHSQSVL FVGLQEHVAK IPLKRCHFHQ TRSACIGAQD PYCGWDAVMK KCTSLEESLS MTQWDQSIPT CPTRNLTVDG SFGPWSPWTP CTHTDGTAVG SCLCRSRSCD RPAPQCGGWQ CEGPRMEITN CSRNGGWTPW TSWSPCSTTC GIGFQVRQRS CSNPTPRHGG RVCVGQNREE RYCNEHLLCP PHVFWTGWGP WERCTAQCGG GIQARRRTCE NGPDCAGSNV EYHPCNTNAC PELKKTTPWT PWTPVNISDN GGHYEQRFRY TCKARLPDPN LLEVGRQRIE MRYCSSDGTS GCSTDGLSGD FLRAGRYSAH TVNGAWSAWT SWSQCSRDCS RGIRNRKRVC NNPEPKFGGM PCLGPSLEFQ ECNILPCPVD GVWSCWSSWS KCSATCGGGH YMRTRSCSNP APAYGGDICL GLHTEEALCN TQTCPESWSE WSDWSVCDAS GTQVRARQCI LLFPVGSQCS GNTTESRPCV FDSNFIPEVS VARSSSVEEK RCGEFNMFHM
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Sema5a Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SEMA5A (Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A))

Alternative Name: Sema5a (SEMA5A Products)

Synonyms: wu:fa95e03 Protein, wu:fi41a04 Protein, SEMAF Protein, semF Protein, 5930434A13 Protein, 9130201M22Rik Protein, AI464145 Protein, Semaf Protein, semaphorin 5A Protein, sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A Protein, semaphorin-5A Protein, SEMA5A Protein, sema5a Protein, Sema5a Protein, LOC100516117 Protein

Background: Bifunctional axonal guidance cue regulated by sulfated proteoglycans, attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1 (By similarity). May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis. {ECO:0000250, ECO:0000269|PubMed:19850054}.

Molecular Weight: 107.3 kDa Including tag.

UniProt: Q62217