alpha 2 Macroglobulin Protein (AA 33-1474) (His tag)

Catalog No. ABIN3135692

Product Details

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Protein Type: Recombinant

Protein Characteristics: AA 33-1474

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This alpha 2 Macroglobulin protein is labelled with His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequence: PQKPIYMVMV PSLLHAGTPE KGCLLFNHLN ETVTVKVSME SVRGNQSLFT DLVVDKDLFH CASFIVPQSS SNEVMFLTVQ VKGPTHEFRR RSTVLIKTKE SLVFAQTDKP IYKPGQMVRF RVVSLDENFH PLNELIPLLY IQDSKKNRIA QWQNFRLEGG LKQLSFPLSS EPTQGSYKVV IRTESGRTVE HPFSVKEFVL PKFEVKVAVP ETITILEEEM NVSVCGIYTY GKPVPGHVTV NICRKYSNPS SCFGEESLAF CEKFSQQLDG RGCFSQLVKT KSFQLKRQEY EMQLDVNAKI QEEGTGVEET GKGLTKITRT ITKLSFVNVD THFRQGIPFV GQVLLVDGRG TPIPYEMIFI GADEANQNIN TTTDKNGLAR FSINTDDIMG TSLTVRAKYK DSNVCYGFRW LTEENVEAWH TANAVFSPSR SFVHLESLPY KLRCEQTLAV QAHYILNDEA VLERKELVFY YLMMAKGGIV RAGTHVLPVT QGHKKGHFSI LISMETDLAP VARLVLYTIL PNGEVVGDTV KYEIEKCLAN KVDLVFHPNI GLPATRAFLS VMASPQSLCG LRAVDQSVLL TKPEAELSAS LVYDLLPVKD LTGFPKGVNQ QEEDTNGCLK QNDTYINGIL YSPVQNTNEE DMYGFLKDMG LKVFTNLNIR KPKVCERLGV NKIPAAYHLV SQGHMDAFLE SSESPTETTR SYFPETWIWD LVIVDSTGVA EMEVTVPDTI TEWKAGAFCL SNDTGLGLSP VIDFQAFQPF FVDLTMPYSV IRGEAFTLKA TVLNYLQTCI RVGVQLEASP DFLATPEEKE QKSHCICMNE RHTMSWAVIP KSLGNVNFTV SAEALDSKEL CRNEVPVVPE RGKKDTIIKS LLVEPEGLEN EVTFNSLLCP TGAEVSEQIS LKLPSDVVEE SARASVTVLG DILGSAMQNT QDLLKMPYGC GEQNMVLFAP NIYVLDYLNE TEQLTQEIKT KAITYLNTGY QRQLNYKHRD GSYSTFGDKP GRSHANTWLT AFVLKSFAQA RRYIFIDESH ITQALTWLSQ QQKDNGCFRS SGSLLNNAMK GGVEDEVTLS AYITIALLEM SLPVTHPVVR NALFCLDTAW KSARRGASGN HVYTKALLAY AFALAGNQDT KKEILKSLDE EAVKEDNSVH WTRAQKPRVP ADLWYQPQAP SAEVEMTAYV LLAYLTTELV PTREDLTAAM LIVKWLTKQQ NSHGGFSSTQ DTVVALHALS KYGAATFTRA KKAAHVTIQS SGAFYTKFQV NNDNQLLLQR VTLPTVPGDY TAKVAGEGCV YLQTSLKYSV LPREKEFPFA LVVQTLPGTC EDLKAHTTFQ ISLNISYIGS RSDSNMAIAD VKMVSGFIPL KPTVKMLERS VHVSRTEVSN NHVLIYLDKV SNQMLTLFFM VQQDIPVRDL KPAIVKVYDY YEKDEFAVAK YSAPCSAGYG NA
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse A2m Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Alternative Name: A2m (A2M Products)

Synonyms: A2MD Protein, CPAMD5 Protein, FWP007 Protein, S863-7 Protein, A2MAC1 Protein, A2m1 Protein, A2maa Protein, Mam Protein, A2mp Protein, A2M Protein, LOC733429 Protein, endod Protein, cpamd5 Protein, fwp007 Protein, s863-7 Protein, a2mb Protein, endodermin Protein, Alpha-2-M Protein, alpha-2-macroglobulin Protein, pregnancy-zone protein L homeolog Protein, alpha-2-macroglobulin S homeolog Protein, pregnancy zone protein Protein, alpha-2-macroglobulin-like Protein, A2M Protein, A2m Protein, LOC477699 Protein, pzp.L Protein, AZL_c00450 Protein, LOC100349077 Protein, a2m Protein, LOC100061656 Protein, LOC100090399 Protein, a2m.S Protein, LOC100469973 Protein, LOC100595735 Protein, PZP Protein, LOC100353095 Protein, LOC101122940 Protein, LOC101801552 Protein, LOC100911545 Protein

Background: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase (By similarity). {ECO:0000250|UniProtKB:P01023}.

Molecular Weight: 161.9 kDa Including tag.

UniProt: Q6GQT1

Pathways: Lipid Metabolism